兔肝VX2移植瘤不同剂量高强度聚焦超声辐照后增殖细胞核抗原的表达及意义  

Expression and Significance of Proliferating Cell Nuclear Antigen in Rabbit Liver VX2 Tumor Model after Irradiation with Different Doses of HIFU

作　　者：欧霞 王雁 邹海蓉 邹建中 李崇雁 

机构地区：[1]重庆市生物医学工程学重点实验室,重庆市超声医学工程重点实验室,省部共建国家重点实验室培育基地,重庆400016

出　　处：《中国医科大学学报》2010年第12期989-991,共3页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30830040,30970767)

摘　　要：目的探讨不同剂量的高强度聚焦超声(HIFU)辐照VX2兔肝肿瘤后增殖细胞核抗原(PCNA)的表达情况及意义。方法 72只荷瘤兔按剂量随机分0W、60W、80W、100W、120W、140W、160W、180W、200W共9个组,单次辐照后24h处死,检测PCNA在VX2肿瘤靶区及肿瘤与正常组织交界处的表达情况。结果随辐照剂量的增加,PCNA在VX2肿瘤靶区阳性表达率逐渐下降(P<0.05),且功率<100W时,阳性表达率与辐照剂量呈线性负相关;PCNA在交界处阳性表达率先上升后下降(P<0.05),但其与辐照剂量间无线性相关性。结论了解HIFU单次辐照剂量与PCNA表达规律之间的关系有利于HIFU治疗选择适宜剂量。Objective To explore the expression and significance of proliferating cell nuclear antigen（PCNA）in rabbit liver VX2 tumor after irradiation with different doses of HIFU.Methods Totally 72 tumor-bearing rabbits were randomly divided into 9 group（s0 W、60 W、 80 W、100 W、120 W、140 W、160 W、180 W、200 W）according to radiation dose.The expressions of PCNA in tumor target and the junction brtween tumor and normal tissues were detected by SABC immunohistochemistry after irritation for 24 h with HIFU and put to death.Results with the radiation dose increased,the positive rate of PCNA decreased in tumor target tissues（P 〈 0.05）,and it was negatively correlated with radiation dose less than 100 W;PCNA positive rate at junction tissues first increased and then decreased,but it had no linear correlation with dose.Conclusion Understanding the relationship between PCNA expression and single dose is helpful to choose a suitable dose for treatment.

关 键 词：高强度聚焦超声 PCNA 抑制 肿瘤 

分 类 号：R318.5[医药卫生—生物医学工程]