西咪替丁增强5-氟脲嘧啶对人胃癌细胞的生长抑制作用  

Cimetidine Enhances the Antiproliferative Effects of 5-fluorouracil on Human Gastric Cancer Cells

作　　者：刘芙蓉[1] 张成宏[1] 高建[2] 李丰[1] 

机构地区：[1]中国医科大学基础医学院细胞生物学教研室,细胞生物学卫生部重点实验室,沈阳110001 [2]中国医科大学实验技术中心,沈阳110001

出　　处：《中国医科大学学报》2010年第12期1021-1023,1027,共4页Journal of China Medical University

摘　　要：目的观察不同浓度的西咪替丁、5-氟脲嘧啶(5-Fu)单用或联合应用对人胃癌细胞MGC-803、BGC-823生长及凋亡的影响。方法用MTT法检测西咪替丁与5-Fu联合应用对胃癌细胞MGC-803、BGC-823存活率的影响;流式细胞仪检测西咪替丁与5-Fu联合应用对胃癌细胞凋亡率的影响。结果西咪替丁与5-Fu联合应用时,西咪替丁在无毒浓度(5～50μmol/L)即可显著增强低浓度5-Fu对胃癌细胞的生长抑制作用。同时还发现,西咪替丁能增强5-Fu诱导胃癌细胞凋亡。结论西咪替丁可提高人胃癌细胞MGC-803、BGC-823对化疗药物5-Fu的敏感性,抑制细胞增殖,促进细胞凋亡。Objective To investigate the effect of cimetidine and 5-fluorouracil（5-Fu）lonely or combination on proliferation and apoptosis of human gastric cancer cells MGC-803,BGC-823 in vitro.Methods MTT assay was used to examine the effect of cimetidine and 5-Fu on the proliferation of MGC-803,BGC-823 cells;flow cytometry was used for estimating apoptosis.Results When cimetidine used in combination with 5-Fu,cimetidine can significantly enhance the antiproliferative effect of lower concentration 5-Fu in no toxic concentrations（5-50 μmol/L）on human gastric cancer cells.Meanwhile it also increased 5-Fu to induce the cells apoptosis.Conclusion Cimetidine can increase the chemosensitivity of 5-Fu in human gastric cancer cells MGC-803,BGC-823 in vitro,inhibit the cells proliferation and promote their apoptosis at higher degree.

关 键 词：西咪替丁 5-氟脲嘧啶 联合应用 胃肿瘤细胞株 

分 类 号：Q279[生物学—细胞生物学]