pap和ipt共表达与提高转基因烟草抗病性的初步研究  

IMPROVED VIRUS RESISTANCE OF TRANSGENIC TOBACCO WITH CO-EXPRESSED pap AND ipt GENES

作　　者：殷娴[1,2] 李朝霞[3] 张海燕 吴国智 程伟[1] 肖尊安[1] 

机构地区：[1]北京师范大学生命科学学院,北京100875 [2]天津市园林花圃,天津300112 [3]中国科学院遗传与发育研究所,北京100101 [4]甘肃亚盛集团博士后科研工作站北京分站,北京100101

出　　处：《北京师范大学学报（自然科学版）》2010年第6期714-718,共5页Journal of Beijing Normal University(Natural Science)

基　　金：国家转基因生物新品种培育科技重大专项基金资助项目(20092X08009-089B)

摘　　要：通过构建pap与ipt共表达的双元载体,利用农杆菌介导,将pap与ipt共表达基因导入烟草K326品种的叶肉细胞,经过抗性筛选和鉴定,获得高表达的转基因植株,并对其进行了攻毒实验.结果表明:pap与ipt共表达基因转化烟草的转化率明显提高(25%),证实转基因细胞中ipt基因表达可降低PAP细胞毒性;接种烟草花叶病毒(TMV)后,pap高表达的转基因植株出现病症的时间延迟20 d,叶片失绿程度较轻,表明该转基因植株对病毒的抗性有一定程度的提高.Agrobacterium tumefaciens harboring a binary vector containing co-expressive genes pap and itp were used to introduce pap and itp into tobacco mesophyll cell K326.Pap-expressing transgenic plants were obtained by selection on Km-containing medium,being confirmed with PCR and RT-PCR.Data showed a transformation efficiency of the two genes at 25%,higher than pap gene alone,indicating that PAP cytotoxicity in transgenic cells was reduced by ipt expression.When the plants were infected with TMV,transgenic plants showed symptoms with a delay of 20 days,with mosaic leaves being mitigated,suggesting that the antiviral ability of transgenic plants was enhanced

关 键 词：美洲商陆抗病毒蛋白(PAP) 异戊烯基转移酶 烟草 烟草花叶病毒 

分 类 号：S572[农业科学—烟草工业]