不同基因型怀地黄组织培养及植株再生研究  

Tissue Culture and Plantlet Regeneration on Different Genotypes of Rehmannia glutinosa

作　　者：赵鸿飞[1] 周延清[1] 姚换灵[1] 傅志远[1] 曹冲[1] 丁婷婷[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《安徽农业科学》2011年第2期673-674,700,共3页Journal of Anhui Agricultural Sciences

基　　金：河南师范大学(国家)大学生创新性实验计划资助项目(2008202);河南师范大学生命科学学院国家级实验教学示范中心资助项目;河南省教育厅自然科学研究计划项目(2011A80022);河南省高校道地药材保育及利用工程技术研究中心开放课题(2010008)

摘　　要：[目的]研究不同基因型怀地黄的组织培养及其植株再生。[方法]以4种不同基因型怀地黄为材料,研究了其在7种不同激素配比培养基上的愈伤组织诱导效应及其分化效果。[结果]7种不同浓度激素配比的培养基均能使4个不同基因型怀地黄产生愈伤组织,且愈伤组织均有不同程度的分化,但不同基因型怀地黄的愈伤组织诱导效应及其分化存在差异,其中武陟6号的愈伤组织诱导及其分化效果较好。[结论]为进一步研究怀地黄的组织培养提供了依据。[Objective]The aim was to study the tissue culture and plantlet regeneration on different genotypes of Rehmannia glutinosa Libosch.f.hueichingensis（Chan et Sehih） Hsiao.[Method]The difference of tissue culture and induction of callus among 4 different kinds of Rehmannia glutinosa cultured on 7 different mediums were studied.[Result]4 varieties of Rehmannia glutinosa cultured on mediums with different concentrations of auxins all could generate callus,and callus had different degrees of differentiation.However,there were differences in callus induction and differentiation of the 4 different genotypes of Rehmannia glutinosa,among them Wuzhi 6 had better callus induction and differentiation.[Conclusion]The research provides reference for further studies on the tissue culture of Rehmannia glutinosa.

关 键 词：怀地黄 组织培养 植株再生 激素配比 

分 类 号：S567[农业科学—中草药栽培]