高纯度银杏黄酮的制备  

Preparation of High-Purity Flavonoids from Ginkgo biloba L.

作　　者：张伟[1] 李芊[1] 林炳昌[1] 

机构地区：[1]辽宁科技大学化学工程学院,辽宁鞍山114051

出　　处：《安徽农业科学》2011年第2期764-765,769,共3页Journal of Anhui Agricultural Sciences

基　　金：辽宁省教育厅团队项目(2008T093);辽宁科技大学2010年度校青年基金团队项目子课题(20100602)

摘　　要：[目的]制备高纯度的银杏黄酮,探索一条规模化制备高纯度银杏总黄酮的工艺路线。[方法]用固相填料为ODS的色谱柱纯化银杏黄酮,考察梯度洗脱、浓缩温度、干燥温度对银杏黄酮含量的影响。[结果]甲醇∶0.5%磷酸=5∶5和7∶3(V∶V)的馏分为目标馏分,最佳浓缩温度和干燥温度为45℃,在最佳条件下,银杏黄酮质量百分含量≥90%,收率为78.3%。[结论]该试验获得了高纯度银杏黄酮,为高纯度的银杏总黄酮制备提供了一种高效的方法。[Objective] To prepare highly purified flavonoids of Ginkgo biloba L.,so as to explore a large-scale preparation process line of high purity flavonoids from Ginkgo biloba L..[Method] Purified with chromatographic column with packing of ODS,effects of mobile phase ratio,concentration temperature and drying temperature on yield of flavonoids from Ginkgo biloba L.were investigated.[Result] The optimum preparation conditions were as follows： target fraction of methanol to 0.5% phosphoric acid ratio of 5∶5 and 7∶3,concentration temperature and drying temperature of 45 ℃.The content of flavonoids from Ginkgo biloba L.was more than 90%,and the yield reached 78.3% under the optimal preparation process.[Conclusion] High-purity flavonoids from Ginkgo biloba L.were obtained,which provided an effective method for the preparation of highly purified Ginkgo biloba L.flavonoids.

关 键 词：银杏总黄酮 高纯度 梯度洗脱 浓缩温度 干燥温度 高效液相色谱法 

分 类 号：S792.95[农业科学—林木遗传育种]