^(99)Tc^m-重组水蛭素的制备及血栓动物模型显像研究  

PREPARATION OF 99 Tc m\|RECOMBINANT HIRUDIN AND STUDY OF IMAGING IN ANIMAL THROMBI MODEL

作　　者：万卫星[1] 张荣军[1] 季顺东[2] 谭成[1] 孙瑞辉[1] 王博诚[1] 韩玉珉[3] 金坚[1] 

机构地区：[1]江苏省原子医学研究所,核医学国家重点实验室无锡214063 [2]苏州医学院血栓与止血研究室,苏州215007 [3]中国科学院生物物理研究所,北京100080

出　　处：《同位素》1999年第3期164-168,共5页Journal of Isotopes

基　　金：卫生部科研基金

摘　　要：采用亚氨基噻吩盐酸盐修饰法制备９９ Ｔｃｍ 重组水蛭素（９９ Ｔｃｍ ｒ Ｈ），并进行小鼠体内分布及兔股动、静脉血栓模型显像分析。结果表明：９９ Ｔｃｍ ｒ Ｈ 的标记率为 ９０８％ ， Ｐ Ｄ１０ 过柱前后放化纯度分别为８８８％ 和９３９％ ，比活度为 ３２９２ Ｇ Ｂｑ／ｇ；，在小鼠心、脑、肝、脾、肺摄取低，肾浓聚高，１５ ｍ ｉｎ达到（６４７２±５．０４）％ Ｉ Ｄ，血清除迅速；兔股动、静脉血栓于 １ ｈ 可清晰显影， Ｔ／ Ｂ 分别为 ２２４ 和１９５。Tc m\|rH is prepared by 2\|iminothiolane modified method. The biodistribution is also determined with 99 Tc m\|rH in mouse, and thrombi imaging is performed in rabbit thrombi model. The results show that the labelling yield of 99 Tc m\|rH is 90.8%, the radiochemistry purity is 88.3% and 93.9% respectively, before and after passing through PD\|10 column. The specific activity is 329.2 GBq/g. The biodistribution in mouse demonstrates that rH is excreted from kidneys ( 64.72±5.04 %ID within 15 min after injection) because of lower uptake in heart, brain, liver, spleen and lungs, and the blood clearance is rapid. The arterial and venous thrombi can be clearly observed within 60 min after injection, T/B ratio is 2.24 and 1.95 respectively.\;

关 键 词：重组水蛭素 凝血酶 显像 血栓 锝99-rH 

分 类 号：R817.42[医药卫生—影像医学与核医学] TQ463.7[医药卫生—放射医学]