丙泊酚对缺氧诱导的大鼠肺泡上皮Ⅱ型细胞凋亡的调控作用  

Effect of propofol on apoptosis induced by hypoxia in rat alveolar type Ⅱ cells

作　　者：何星颖[1] 石学银[1] 袁红斌[1] 徐海涛[1] 宁慧杰[1] 

机构地区：[1]第二军医大学附属长征医院麻醉科,上海市200003

出　　处：《临床麻醉学杂志》2010年第11期963-965,共3页Journal of Clinical Anesthesiology

基　　金：第二军医大学附属长征医院"三重三优"基金

摘　　要：目的探讨丙泊酚预处理对缺氧条件下大鼠Ⅱ型肺泡上皮(ATⅡ)细胞凋亡的影响。方法体外分离并培养大鼠ATⅡ细胞,将细胞分为三组:缺氧组(H组)、丙泊酚-缺氧组(P组)和对照组(C组)。测定各组细胞存活率、早期凋亡率、缺氧诱导因子-1α(HIF-lα)mRNA及Bnip3LmRNA表达水平。结果与C组比较,H组和P组细胞存活率显著降低(P<0.05)、早期凋亡率显著升高(P<0.05),HIF-1αmRNA及Bnip3LmRNA表达显著增强(P<0.05)。与H组比较,P组上述指标显著降低(P<0.05)。结论丙泊酚预处理可抑制缺氧条件下大鼠ATⅡ细胞凋亡,推测其与丙泊酚-HIF-1α-缺氧反应元件(HRE)轴的抑制有关。Objective The purpose of this study was to investigate the effect of propofol on apoptosis of alveolar type Ⅱ （ATⅡ） cells induced by hypoxia.Methods cultured ATⅡ cells were divided into three groups：control group（group C）,hypoxia group（group H） and hypoxia-propofol group （group P）. Cell survival rate,apoptosis rate,hypoxia-induced factor-1α （HIF-1α） mRNA and Bnip3L mRNA were determined in each group.Results Compared with that of group C,cell survival rate significantly decreased while apoptosis rate,HIF-lα mRNA and Bnip3L mRNA expression significantly increased in group H. Propofol pretreat improved apoptosis-related index remarkably.Conclusion Propofol pretreatment exert anti-apoptosis effect on ATⅡ cells,which might be related with inhibition of propofol-HIF-1α-HRE axis.

关 键 词：丙泊酚 Ⅱ型肺泡上皮细胞 凋亡 

分 类 号：R965[医药卫生—药理学]