黄芪提取物对大鼠局灶性脑缺血再灌注血脑屏障损伤的影响  

Effect of Extract of Astragalus on Alterations of Blood Brain Barrier Induced by Local Cerebral Ischemia-reperfusion Injury

作　　者：黄茸茸[1] 李卫平[2] 李维祖[2] 

机构地区：[1]安徽新华学院药学院,合肥230088 [2]安徽医科大学基础医学院药理教研室,合肥230038

出　　处：《中国现代应用药学》2010年第12期1064-1068,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：安徽省十五攻关项目(001803016);安徽省自然科学基金资助项目(00044416)

摘　　要：目的研究黄芪提取物(extract of astragalus,EA)对大鼠局灶性脑缺血(MCAO)再灌注的血脑屏障损伤的影响。方法采用线栓法制备MCAO再灌注模型,观察EA对缺血再灌注大鼠的神经功能障碍、脑梗死体积和脑含水量、外周血循环内皮细胞(CEC)含量、缺血脑组织中细胞间粘附分子(ICAM-1)的表达、缺血侧脑组织皮质血脑屏障超微结构的病理学变化的影响。结果 EA80 mg.kg·1,EA(40,80 mg.kg·1)、EA(20,40,80 mg.kg·1)可分别改善缺血再灌注2,8,24 h大鼠的神经功能障碍;能明显降低外周血中的CEC含量,明显减轻缺血脑组织的脑水肿和脑梗死体积,对脑缺血再灌注后血脑屏障超微结构的病理改变有一定的改善作用;能减少ICAM-1免疫反应阳性血管数。结论 EA对局灶性脑缺血再灌注损伤大鼠有一定的保护作用,作用机制可能与其保护血管内皮细胞,减轻脑缺血再灌注后的血脑屏障损伤有关。OBJECTIVE To study the effect of extract of astragalus（EA） on alterations of blood brain barrier after local cerebral ischemia-reperfusion injury in rats.METHODS Middle cerebral artery occlusion（MCAO） was used to make local cerebral ischemia-reperfusion model with intravascular nylon filament occlusion.The neurological function score,brain edema and infarct volume,the counts of peripheral blood circulating cells,the expression of intercellular adhesion molecule-1,pathological changes in blood-brain barrier ultrastructure in rats after focal cerebral ischemia-reperfusion were observed.RESULTS EA 80 mg·kg-1 decreased the neurological function score;EA（40 and 80 mg·kg-1） reduced brain edema and infarct volume,the counts of peripheral blood circulating cells,pathological changes in blood-brain barrier ultrastructure;EA（20,40 and 80 mg·kg-1） decreased the expression of intercellular adhesion molecule-1 in brain tissue.CONCLUSION EA has protective effects against cerebral ischemia reperfusion injuries,the mechanism may be related with reducing BBB injury induced by local cerebral ischemia-reperfusion.

关 键 词：黄芪提取物 局脑缺血 再灌注损伤 血脑屏障 ICAM-1 血循环内皮细胞 

分 类 号：R285.5[医药卫生—中药学]