PCR-ELISA法检测HBV感染者精液HBV-DNA的评价  

Evaluation of PCR-ELISA for detection of HBV-DNA in semen

作　　者：黄伟佳[1] 钟剑峰[1] 高兴成[1] 岳巍巍[1] 

机构地区：[1]广州医学院第三附属医院,广州510150

出　　处：《中国男科学杂志》2010年第12期18-20,23,共4页Chinese Journal of Andrology

基　　金：广东省医学科学研究基金项目(项目编号:A2008296)

摘　　要：目的评价PCR—ELISA法检测HBV感染者精液中HBV—DNA检出情况及其临床意义。方法选取男性HBV感染者56例，采集患者精液、静脉血，用PCR．ELISA法来检测患者精液及血清中HBV—DNA。标本先行PCR扩增，之后行杂交固定，酶联反应后行显色、最后450nm下读光密度值（O．D），再用HBV—PCR定量的计算公式用计算样本中HBV病毒含量（单位：拷贝／m1）。结果56例精液标本HBVDNA阳性10例，阳性率17．85％，拷贝数为1．317×103-6．351×10^3／ml，平均拷贝数为4．617×10^4／ml。56例血清标本阳性49例，阳性率为87．50％，拷贝数1．432×10^3-5．215×10^7／ml，平均拷贝数为2．462×10^6／ml。结论PCR—ELISA法定量检测精液中HBV—DNA具有较强的特异性和灵敏度，为临床了解乙型肝炎患者精液中肝炎病毒的感染状态提供了帮助，具有重要的临床意义。Objective To evaluate the clinical value of PCR-ELI S A for detection of HBV -DNA in semen. Methods Semen samples and serum samples from 56 HBV male patients were collected respectively. HBV-DNA in samples were detected by quantitative PCR-ELISA. Results Ten of 56 semen samples had positive results of HBV- DNA. The positive rate was 17.85%. The copy number of HBV-DNA was 1.317 ×10^3 ~6.351×10^5/ml. The average copy number was 4.617 ×10^4/ml. In 56 serums samples, there were 49 positive samples for HBV-DNA. The positive rate was 87.50%. The copy number of HBV-DNA was 1.432 ×10^3~5.215 ×10^7/ml. The average copy number was 2.462 ×10^6/ml. Conclusion Quantitative PCR-ELISA showed high sensitivity and specificity for detection of HBVDAN in semen. It will help to monitor the state of HBV- DNA in semen.

关 键 词：酶联免疫吸附测定 聚合酶链反应 肝炎病毒 乙型 精液 

分 类 号：R446.144[医药卫生—诊断学]