正交试验优化复方枳参胶囊处方药材的提取工艺  

Optimization of extract technique of Fufang Zhishen Capsule through orthogonal experiment

作　　者：阙慧卿[1] 林绥[1] 邓思珊[1] 钱丽萍[1] 郭舜民[1] 齐一萍[1] 

机构地区：[1]福建省医学科学研究所福建省医学测试重点实验室,福建福州350001

出　　处：《现代药物与临床》2010年第6期457-459,共3页Drugs & Clinic

基　　金：福建省省属科研院所公益类基本科研专项(2009R10017-4)

摘　　要：目的研究影响复方枳参胶囊提取效果的各种因素,确定最佳提取工艺。方法采用正交试验设计,以浸膏收率和槲皮素提取率为指标,考察回流提取时间(A)、乙醇体积分数(B)、提取次数(C)和乙醇用量[乙醇体积(mL)/药材量(g),D]4个因素对提取效果的影响,优选出复方枳参胶囊的提取工艺。结果在所考察的4个因素中,对复方枳参胶囊提取效果的影响程度依次为:B>D>C>A,即乙醇体积分数>乙醇用量>提取次数>回流提取时间,确定出最佳提取工艺为B2D3C2A3,即10倍于药材量(g)的70%乙醇(mL),回流提取2次,每次2h为最佳提取工艺。结论本实验优选出的提取工艺适用于复方枳参胶囊的提取。Objective To determine the best extraction technique by studying the different factors that might affect the extraction of Fufang Zhishen Capsules.Methods The orthogonality experiment was adopted in the study.Extraction rate of quercetin and the extractum rate were taken as indices.The effects of four factors on the extraction were studied,including reflux extraction time（A）,volume grade of alcoho（lB）,extraction times（C）,and alcohol volume（D）which was calculated by alcohol volume（mL）divided by the amount of materials（g）.On the basis of the study on the four factors,the extraction technique was optimized.Results The effects of the four factors were listed as follows：B〉D〉C〉A.The best extraction technique was described as B2D3C2A3.The amount of 70% alcohol was 10 times of the medicinal materials.There were total 2 times of reflux extraction with 2 hour in each time.Conclusion The optimized technique is suitable to the extraction of Fufang Zhishen Capsule.

关 键 词：复方枳参胶囊 槲皮素 正交试验 提取工艺 高效液相色谱 

分 类 号：R943[医药卫生—药剂学]