化橘红黄酮类生物合成中功能基因的克隆与序列分析  

Cloning and Sequence Analysis on Functional Genes of Flavonoid Biosynthesis in Citrus grandis 'tomenxosa'

作　　者：文海涛[1] 赵红英[1] 林励[1] 方桂芳[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《中药材》2010年第11期1686-1689,共4页Journal of Chinese Medicinal Materials

基　　金：国家发改委高科技专项(2003-26)

摘　　要：目的:克隆获得广东道地药材化橘红黄酮类化合物生物合成中几个重要功能基因。方法:利用CTAB-LiCl法提取高质量的化橘红总RNA,采用RT-PCR技术克隆基因。结果:克隆获得了化橘红黄酮类生物合成中三个重要功能基因,它们分别为查尔酮合成酶基因,该基因编码区全长1173 bp,编码391个氨基酸,NCBI序列登录号:GQ892059;黄烷酮-3-羟化酶基因,该基因编码区全长1 086 bp,编码362个氨基酸,登录号:GU323284;查尔酮异构酶基因,该基因全长666 bp,编码222个氨基酸,登录号:GU323285。结论:CTAB-LiCl法能提取高质量的化橘红总RNA,克隆获得的三个功能基因都具有开放阅读框。Objective：Cloned several important functional genes from Citrus grandis ＇tomentosa＇,which was the genuine medicinal materials from Guangdong province.Methods： CTAB-LiCl treatment was used to extract RNA from Citrus grandis ＇tomentosa＇,the technology of RT-PCR was used to clone the genes.Results： Three genes were cloned from Citrus grandis ＇tomentosa＇,which were Chalcone Synthase（CHS） gene,Flavanone 3-hydroxylase（F3H） gene and Chalcone isomerase（CHI） gene.The open reading frame（ORF） of CHS gene was consisted of 1173 bp,and encoded 391 amino acid residues,that of F3H gene was consisted of 1086 bp,and encoded 362 amino acid residues,that of CHI gene was consisted of 666 bp,and encoded 222 amino acid residues.The accession number of three genes were GQ892059,GU323284 and GU323285 respectively.Conclusion： CTAB-LiCl treatment could extract high quality RNA from Citrus grandis＇tomentosa＇,which could be used to follow-up work..The three functional genes which were cloned from Citrus grandis ＇tomentosa＇ had ORFs.

关 键 词：化橘红 黄酮类 功能基因 查尔酮合成酶 黄烷酮-3-羟化酶 查尔酮异构酶 

分 类 号：Q781[生物学—分子生物学]