RP-HPLC法测定大鼠血浆中补阳还五汤及总苷中芍药苷的含量及药代动力学研究  

Determination of the Concentration of Paeonflorin of the BYHWD and Total Glycoside in the Plasma of Rats by Reverse Phase High Performance Liquid Chromatography and Studies on It′s Pharmacokinetics

作　　者：邓凯文[1] 贺福元[2] 刘文龙[2] 

机构地区：[1]湖南中医药大学第一附属医阮针灸科,湖南长沙410007 [2]湖南中医药大学中药药性与药效安验室,湖南长沙410007

出　　处：《中药材》2010年第11期1768-1770,共3页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(30472199,81073142);国家中医药管理局中医药科研基金(02-03ZP41)

摘　　要：目的：对大鼠血浆补阳还五汤及总苷中芍药苷的含量进行了测定,并对其药代动力学研究;方法：采用反相高效液相色谱法测定,其条件为色谱柱：C18,4.6×250 mm,5μm;检测波长：230 nm;流动相：甲醇∶水（33∶67）,流速：1 mL/min;温度：35℃。药代动力学参数采用DAS软件处理。结果：血浆样品中芍药苷线性范围为在（0.8010~80.10）μg/mL,r=0.9999,回收率为99.77%,RSD为2.183%。芍药苷药代动力学参数：复方：t1/2（α）为2.569 min;t1/2（β）为64.53 min;K12为0.1457 min-1;K21为0.01767 min-1;K10为0.2366 min-1;总苷：t1/2（α）为1.829 min;t1/2（β）为72.27 min;K12为0.1587 min-1;K21为0.01708 min-1;K10为0.2127 min-1。结论：该法快速、简便,准确。补阳还五汤复方及总苷中芍药苷药物动力学为二室模型。中药复方成分组合对药代动力学参数有一定影响。Objective：A RP-HPLC method for the determination of paeonflorin of the BYHWD and total glycoside in the plasma of white rat was reported.The pharmacokinetics of paeonflorin of the BYHWD in white rat were studied.Methods：The RP-HPLC was used with the conditions that the column was C18,4.6×250 mm,5 μm;the detection of wavelengths was set at 230 nm;A solution of methanol-water（33∶67）was used as the mobile phase;The mobile velocity was 1 mL/min;the pressure of column was（20.71±0.4173） MPa;the temperiture was 35 ℃.The parameters of pharmacokinetics was dealt with DAS.Results ：The calibration curve was linear in the range of（0.8010～80.10） μg/mL,with r=0.9999;The average recovery is 99.77%;RSD is 2.183%;The pharmacokinetic parameters of the paeonflorin was that in the compound prescripition,t1/2（α） was 2.569 min;t1/2（β） was 64.53 min;K12 was 0.1457 min-1;K21 was 0.01767 min-1;K10 was 0.2366 min-1;in the total glycoside,t1/2（α） was 1.829 min;t1/2（β） is 72.27 min;K12 was 0.1587 min-1;K21 was 0.01708 min-1;K10 was 0.2127 min-1;Conclusions：The method is rapid,simple and easy to use in determination of Paeonflorinof the BYHWD in the plasma of Mice.The pharmacokinetics of paeonflorin of the BYHWTD in the mice abidied by two compartmental model,as difference as constitution.

关 键 词：补阳还五汤 总苷 芍药苷 RP-HPLC 药代动力学 

分 类 号：R285.5[医药卫生—中药学]