应用参考方法对血清γ-谷氨酰基转移酶的测量不确定度评定  

Apply Reference Procedure to Evaluate the Measurement Uncertainty of Serum Gamma-glutamyltransferase

作　　者：倪红兵[1] 施秀英[1] 王惠民[1] 肖静[2] 鞠少卿[1] 王跃国[1] 

机构地区：[1]江苏南通大学附属医院检验医学中心,江苏南通226001 [2]江苏南通大学公共卫生学院,江苏南通226019

出　　处：《现代检验医学杂志》2010年第6期51-54,共4页Journal of Modern Laboratory Medicine

基　　金：江苏省医学重点学科建设资助项目（XK200723）,南通大学引进人才科研启动基金（项目编号：03080135）.

摘　　要：目的 探讨临床实验室对测量不确定度的评定方法和程序.方法 应用国际临床化学联合会（IFCC）公布的参考方法对参考实验室外部质量评价计划（RELA）样本进行γ-谷氨酰基转移酶（GGT）催化活性浓度测定,明确反映整个过程测量不确定度分量的来源,按A,B两类不确定度的计算方式对各分量分别进行评定,计算合成标准不确定度,最后取95%置信区间,计算出扩展不确定度.结果 GGT催化活性浓度均值为3.492 μkat/L,合成标准不确定度为0.032 μkat/L,取95%置信区间,包含因子k=2,则扩展不确定度（U）为0.064 μkat/L.结论 临床实验室的测量不确定度与传统的测量误差相比,更能反映测量的水平,对临床检验工作有一定的指导意义.Objective To explore the method and procedure for evaluation of measurement uncertainty in clinical laboratory. Methods The concentration of gamma-glutamyltransferase （γ-GT,GGT） of the sample of external quality assessment program for reference laboratories （RELA） was measured by the reference procedure of IFCC;the sources of each uncertainty component in whole procedure of analysis were identified ;evaluation of the uncertainty components was processed by using two types of method and then a combined standard uncertainty was calculated, finally an expanded uncertainty was given according to 95% confidence interval. Results The average catalytic activity concentration of GGT was 3. 492μtkat/L,the related combined standard uncertainty was 0. 032 μkat/L,the 95% confidence interval was taken,the coverage factor （k） was 2,so the expanded uncertainty was 0. 064 μkat/L. Conclusion Compared with the traditional measurement errors, the measurement uncertainty in clinical laboratory could reflect the measurement ability and have instructive significance for routine clinical laboratory.

关 键 词：测量不确定度 Γ-谷氨酰基转移酶 合成标准不确定度 扩展不确定度 

分 类 号：R446.112[医药卫生—诊断学]