ABX Pentra 120全自动血液分析仪复检规则的制定及评价  

Formulation and Evaluation of Hematology Review Criteria for ABX Pentra 120 Automated Hematology Analyzer

作　　者：张善辉[1] 巫炀[1] 王平平[1] 郭丽[1] 

机构地区：[1]姜堰市人民医院检验科,江苏姜堰225500

出　　处：《现代检验医学杂志》2010年第6期94-95,98,共3页Journal of Modern Laboratory Medicine

摘　　要：目的 建立ABX Pentra 120全自动血液分析仪的复检规则,保证血细胞分析结果的准确性.方法 根据ABX Pentra 120全自动血液分析仪的工作性能,参照国际41条自动血细胞分析和分类复检规则,并结合姜堰市人民医院实际制定出复检规则.600份EDTA-K3抗凝血,在2 h内用ABX Pentra 120 全自动血液分析仪进行检测,同时制备血涂片,瑞氏染色后在显微镜下观察红细胞、白细胞、血小板的数量及形态.结果 自动通过复检规则确认的样本469份,占78.2%,需用显微镜法复检的样本131份,占21.8%.仪器检测结果和镜检结果比较,真阳性率为7.0%,假阳性率为14.8%,真阴性率为75.0%,假阴性率为3.2%.假阴性的结果主要集中于有核红细胞及红细胞、血小板的形态.结论 3.2%的假阴性率符合国际血液学复审协作组关于假阴性率小于5%的规定.复检规则的应用,可提高检验结果的准确性,复检规则适合姜堰市人民医院的情况.Objective To formulate and evaluate the hematology review criteria for ABX Pentra 120 automated analyzer. Methods On the basis of ABX Pentra 120 operating characteristic,referring to 41 consensus rules,the review rules for Jiangyan People＇s Hospital were established. 600 blood samples collected from with EDTA-Ks anticoagulation were examined by ABX Pentra 120 automated analyzer within two hours. While,a peripheral blood smear of every sample was prepared. The cell with wright＇s staining was examined under the microscope. The amount and the form of the cell was observed. Results 469 samples passed the rules automatically,the rate was 78.2%. 131 samples should be reviewed by microscopic determination, the rate was 21.8 %. Compared with the positive results obtained by microscopic examination,analyzer presented 7.0% of true positive rate,14.8% of false positive rate,75.0% of true negative rate,3.2% of false negative rate. The false negative mostly created by identification of nucleated red blood cell and the morphology of red blood cell and platelet. Conclusion 3.2% of false negative rate was less than 5.0% ,which was consistent with the criteria instituted by the international consensus group for hematology review.Byusing of review criteria,the accuracy of measurement can be better enhanced. The criteria should be feasible to the practice.

关 键 词：血细胞分析仪 复检规则 显微镜镜检 

分 类 号：R446.111[医药卫生—诊断学]