低温胁迫对野牛草幼苗渗透调节物与根系活力的影响  

Effect of Low Temperature Stress on Osmotic Regulation Substances and Root Vitality in Buffalograss Seedling

作　　者：康俊梅[1] 李燕[1] 沈静[2] 熊军波[1] 杨青川[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]甘肃农业大学,兰州730070

出　　处：《中国畜牧兽医》2010年第12期18-22,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划农林动植物育种工程项目子课题"优质抗逆专用草新品种选育"(2006BAD01A19-3);国家科技支撑计划子课题"聚合多目标性状的高效育种技术"(2008BADB3B04-1)

摘　　要：以暖季型草坪草"中坪一号"野牛草为材料,研究低温胁迫对其幼苗渗透调节物与根系活力的影响。结果表明,渗透调节物与野牛草幼苗低温调节机制密切相关。在低温胁迫下,可溶性糖、可溶性蛋白质、游离脯氨酸含量呈上升的趋势。随着低温处理时间的延长,可溶性糖含量的增加趋于平缓;可溶性蛋白质含量受低温影响较大,随低温胁迫强度的增大,叶片中可溶性蛋白质含量明显增加;游离脯氨酸含量的变化较敏感,低温处理初期,脯氨酸含量急剧上升,随着处理时间延长脯氨酸含量又缓慢降低,但整个过程中脯氨酸含量保持在较高水平;低温胁迫对野牛草幼苗根系活力的影响较显著,随着低温胁迫时间的延长,根系活力急剧降低,说明根系对维持植物正常生长发育的重要性,或可作为植物抗寒性鉴定的一项重要指标。Using the warm-season turfgrass ＇Zhongping No.1＇ buffalograss as experimental material,effects of low temperature stress on osmotic regulation substances and root vitality were studied.The results showed that osmotic regulation substances closely related to the cold acclimation adjustment mechanism of buffalograss seedlings under low temperature stress.With the prolonged of the treatment,contents of soluble sugar and protein as well as praline increased.But the content of soluble sugar increased gently at the later stage;the content of soluble protein did markedly at all time.This displayed soluble protein maybe played an important role in adjustment mechanism under low temperature.Then change of proline content was sensitive to cold stress.And proline content sharply increased in the earlier stage and slowly decreased again with the extension of stress time.However,the content of proline remained at a relatively high level throughout the course of cold stress.Meanwhile root vitality of buffalograss seedlings was affected largely by low temperature.The root vitality decreased quickly under low temperature stress.This demonstrated the root system was important to maintain normal growth and development of plants,and it maybe was looked upon as an important indicator for identification of cold tolerance of plants.

关 键 词：低温胁迫 野牛草 渗透调节物 根系活力 

分 类 号：S643[农业科学—蔬菜学]