一氧化氮对猪腔前卵泡颗粒细胞凋亡的影响  

Effects of Sodium Nitro-prusside on the Granulosa Apoptosis Rate of Porcine Preantral Follicles

作　　者：权青[1,2] 陶勇[2] 章孝荣[2] 汪金菊[2] 姚桂东[3] 

机构地区：[1]安徽农业大学经济技术学院,合肥230036 [2]安徽农业大学动物科技学院,合肥230036 [3]合肥市妇幼保健医院生殖中心,合肥230001

出　　处：《中国畜牧兽医》2010年第12期124-127,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30600432);安徽优秀青年科技基金(06041081);院青年教师基金(JJ2009007)

摘　　要：本试验旨在研究在培养液中添加不同浓度的NO供体硝普钠(sodium nitro-prusside,SNP)(0、0.001、0.01、0.1、1.0 mmol/L)处理,研究培养4 d后的腔前卵泡颗粒细胞凋亡情况。结果表明,1.0 mmol/L SNP组凋亡率显著高于其他各组(P<0.05),而0.001 mmol/L SNP组凋亡率显著低于其他各组(P<0.05)。说明高浓度的SNP对猪腔前卵泡颗粒细胞凋亡起促进作用,低浓度的SNP起抑制作用。In order to understand the effects of nitric oxide（NO） on granulose apoptosis of porcine preantral follicles at four days of the culture,we treated the follicles with different concentrations of sodium nitro-prusside（0,0.001,0.01,0.1 and 1.0 mmol/L SNP）,a nitric oxide donor.The results indicated that 1.0 mmol/L SNP was significantly higher than other treatments（P0.05）,0.001 mmol/L SNP was significantly lower than other treatments（P0.05）.In conclusion,high concentrations of SNP in culture medium can significantly stimulate follicles granulose apoptosis,low concentrations of SNP inhibit follicles granulose apoptosis.

关 键 词：猪 腔前卵泡 颗粒细胞凋亡 

分 类 号：S852.163[农业科学—基础兽医学]