免疫组化法检测布氏杆菌在人工感染牛体内的分布  

Detection of Brucellosis in Experimentally Infected Cattles with Immunohistochemical Method

作　　者：李勇[1,2] 夏志平[2] 刘雯[1,2] 张宁[1,2] 李志萍[2] 胡乐鹏[2] 屈雪琪[1] 白雪[1] 梅林[1] 高英杰[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]中国人民解放军军事医学科学院军事兽医研究所,长春130062

出　　处：《中国畜牧兽医》2010年第12期232-234,共3页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技重大专项课题(2009ZX10605)

摘　　要：将布氏杆菌分不同剂量皮下注射接种于健康牛,于接种后45 d剖杀,采集相关的器官组织制备组织切片,应用免疫组化方法检查细菌在牛体内的分布。结果显示,肝脏、脾脏、肾脏、淋巴结出现强阳性信号;在高剂量注射的牛体内,肺脏、颌下淋巴结出现阳性细胞;心脏和健康牛组织呈阴性反应。阳性信号主要位于感染细胞的胞质中,偶尔出现在细胞核内。The healthy conventional cattles were inoculated subcutaneouly with different CFU of Brucella abortus strain,the cattles were necropsy on day post inoculation.Tissues were collected for preparing tissue sections and immunohistochemistry protocol was developed to detect distribution of B.abortus bacterium in tissue samples.Results showed that in liver,spleen,kidney and lymphoid tissues were positive for B.abortus antigen.Positive signals also presented in lung,mandibular lymph node.Other tissues such as heart and control were negative for antigen.B.abortus antigens were localized predominately in the cytoplasm of the infected cells and rarely observed in the nucleus of the cells.

关 键 词：布氏杆菌 免疫组化 阳性信号 

分 类 号：S854.4[农业科学—临床兽医学]