高效液相色谱法测定消咳喘片的含量  

Determination of dahuyian rhododendron leaf oil in Xiaokechuan tablets by HPLC

作　　者：宋迎权 刘鹏 段祥伟[3] 

机构地区：[1]哈药集团制药六厂技术部,150056 [2]哈药集团制药六厂五车间 [3]哈尔滨商业大学药学院

出　　处：《中国现代药物应用》2010年第24期20-21,共2页Chinese Journal of Modern Drug Application

摘　　要：目的建立高效液相色谱法测定消咳喘片中有效成分满山红油的含量。方法采用Shi-madzuVP-ODS(150mm×4.6mm,5μm)色谱柱。流动相:氯仿:水:乙醚(25:22:53),体积流速:1.0ml/min,检测波长:300nm,进样量:10μl。结果满山红油在0.15～0.60μg之间线性关系良好(r=0.9995),平均回收率为100.63%,RSD=0.35%(n=9)。结论本方法简便易行,结果准确可靠,适用于消咳喘片含量的测定。Objective To establish an HPLC method for determination of Dahurian Rhododendron Leaf Oil in Xiaokechuan tablets.Methods Samples were separated on Shimadzu VP-ODS column（150 mm×4.6 mm,5μm）using chloroform-water-ethylether（25：22.5：52.5）as the mobile phase at a flow rate of 1.0 ml/min.The detective wavelength was set at 300 nm and the sample size was 10 μl.Results The Dahurian Rhododendron Leaf Oil had good liner relationship in 0.15~0.60 μg（r=0.9995）,and the average recovery was 100.63%,RSD=0.35%（n=9）.Conclusion This method is simple,rapid,accurate and precise.

关 键 词：消咳喘片 满山红油 高效液相色谱法 含量测定 

分 类 号：R286[医药卫生—中药学]