一株耐盐好氧反硝化细菌的分离鉴定及生长特性研究  

Isolation and Identification of a Salt-Tolerant Aerobic Denitrifier and Study on Its Growth Characteristics

作　　者：吐尔逊阿依.吾甫尔 史应武[2] 王纯利[1] 马晶[2] 

机构地区：[1]新疆农业大学草业与环境科学学院,乌鲁木齐830052 [2]新疆农业科学院微生物应用研究所,乌鲁木齐830091

出　　处：《新疆农业科学》2010年第11期2216-2220,共5页Xinjiang Agricultural Sciences

基　　金：新疆特殊环境微生物实验室开放课题(xjys0203-2009-02)

摘　　要：【目的】从乌鲁木齐10号温泉的沉积物中筛选出反硝化作用较强的菌株,并对该菌株的生物学特性进行系统的研究。【方法】用稀释法,从沉积物中分离纯化了28株反硝化细菌,通过Giltay液体培养筛选出反硝化作用较强的一株菌NSA4。对该细菌进行形态观察、生理生化及最适生长条件测定,在此基础上,进一步分析16S rDNA基因序列,确定了分离菌株的分类学和系统发育地位。【结果】在接种NSA4菌株的试管内5 d后培养基开始变蓝,并有小量气体产生,滞留在小试管中;7 d后培养基由绿色变为蓝色,小试管内的气体明显增多;该菌为革兰氏阴性杆菌。其最适生长温度为30℃,最适生长pH为7,经PCR扩增后测定该菌株的16SrDNA基因序列表明,由16S rDNA序列比较可知该菌与Pseudomona brassicacearum同源性达99%。【结论】这一耐盐反硝化细菌可以用于新疆硝酸盐污染农田土壤与地下水的修复。[ Objective and Method] To isolate strong denitrifiers from hot spring sedimentary soil collected in Urumqi NO. 10 Hot spring and systematically study their biological characteristics. [ Result] Twenty eight denitrifiers were isolated by using Giltay medium, of which one isolate NSA4 showed strong denitrifying ability, produced gas earlier and faster than other isolates. NSA4 is a Gram negative short rod bacterium. The optimal growth temperature and pH value are 30℃ and 7 respectively for the isolate. The 16S rDNA sequence was compared with the published data in Genbank by using BLAST. The homology with Pseudomona brassicacearum is 99%. [ Conclusion]Such salt - tolerant denitrifier might be used for the remediation of nitrate polluted soil and groundwater in Xinjiang.

关 键 词：反硝化细菌 分离鉴定 硝酸盐 反硝化作用 

分 类 号：S188[农业科学—农业基础科学]