栗蚕基因组DNA提取方法比较试验  

Comparison of DNA Extraction Method for Dictyoploca japonica Moore Gene Group

作　　者：叶青雷[1] 李志[1] 焉鹏娟[1] 赵淑英[1] 任淑文[1] 范娟[1] 谢忠秋[1] 王东风[1] 

机构地区：[1]黑龙江省蚕业研究所,哈尔滨150086

出　　处：《北方蚕业》2010年第4期22-23,28,共3页North Sericulture

摘　　要：栗蚕蛹中含有大量的脂肪和蛋白质类物质,这些物质易与DNA结合形成粘稠的胶状物,影响栗蚕基因组DNA的提取。针对上述问题,本研究采用十二烷基硫酸钠-蛋白酶K(SDS-PK)消化法和十六烷基三甲基溴化铵(CTAB)法提取栗蚕基因组总DNA进行比较试验。结果表明:CTAB法提取的DNA产量高;SDS-PK法提取的DNA质量好。The chrysalis of Dictyoploca japonica Moore contains abundant substances such as fat and protein,which are easily combined with DNA to form sticky substances which affect the extraction of DNA gene group. Aiming at the above problem,the research applied the methods of SDS-PK Digestion and CTAB to extract the total DNA gene group to conduct the comparison experiment. The results showed that CTAB had a high yield of DNA extraction; while SDS-PK can extract good quality DNA.

关 键 词：栗蚕 DNA提取 SDS-PK法 CTAB法 

分 类 号：S885[农业科学—特种经济动物饲养]