T-bet和Gata-3基因在脐带血中的表达特点  

Expression feature of T-bet and Gata-3 transcription factors on umbilical cord blood

作　　者：林锦绒[1] 陈少华[2] 李萡[2] 杨力建[2] 李扬秋[2] 

机构地区：[1]暨南大学第一附属医院妇产科 [2]暨南大学医学院血液病研究所,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2010年第6期569-571,共3页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(30972455);中央高校基本科研业务费专项资金项目(21610408)

摘　　要：目的:了解转录因子T-bet和Gata-3基因在脐带血中的表达情况。方法:采用实时定量RT-PCR检测21例脐带血单个核细胞(CBMC)中转录因子T-bet和Gata-3基因的表达水平,21例健康成人外周血单个核细胞(PBMC)作为对照。结果:T-bet基因和Gata-3基因在CBMC和PBMC中均存在一定表达,Gata-3基因在CBMC中的表达水平(0.43±0.35)%与PBMC(0.42±0.27)%相比无明显差异(P=0.925),而T-bet基因在CBMC中的表达水平(0.47±0.48)%则明显低于健康成人组(2.12±1.48)%(P=0.000)。结论:CBMC中T-bet基因表达低下,可能与脐带血移植后GVHD发生率低下以及发生程度较轻有关。Aim：In order to evaluate the expression level of transcription factors T-bet and Gata-3 mRNA in umbilical cord blood from humans. Methods： Real-time PCR was used for detecting T-bet and Gata-3 genes expression level in 21 cases of mononuclear cells from umbilical cord blood（CBMC）.21 cases of peripheral blood mononuclear cells（PBMC） of healthy adults served as controls.Results： T-bet and Gata-3 genes were expressed in all cases from both CBMC and PBMC.The expression level of Gata-3 gene（0.43%±0.35%） in CBMC was not significant difference in comparison with PBMC（0.42%±0.27%）（P=0.925）.Compared with PBMC（2.12%±1.48%）,the expression level of T-bet gene from CBMC was obviously down-regulated（P=0.000）.Conclusion：The down-regulated expression of T-bet gene might relate to the reduced incidence and severity of GvHD after allogeneic transplantation of umbilical cord blood.

关 键 词：T-BET基因 Gata-3基因 脐带血 实时定量RT-PCR 

分 类 号：R392.11[医药卫生—免疫学]