电纺羧甲基壳聚糖复合rhBMP-2生长因子纤维膜的制备  

Preparation of Carboxymethy Chitosan Compound with growth factor based Fibers Membrane with Emlectrospinning

作　　者：唐涛[1] 谢彤[1] 齐宏旭[2] 胡平[2] 

机构地区：[1]清华大学医院口腔科,副主任医师北京100084 [2]清华大学化工系,博士北京100084

出　　处：《中华老年口腔医学杂志》2010年第4期243-246,共4页Chinese Journal of Geriatric Dentistry

基　　金：清华-裕元医学科学研究基金项目(2002400575)

摘　　要：目的:利用静电纺丝技术以羧甲基壳聚糖复合生长因子(rhBMP-2)缓释微球制备牙周组织再生引导膜。方法:将载有生长因子的钙藻酸盐微球与主体羟基磷灰石(CCS/nHA)羧甲基壳聚糖混合利用静电纺丝技术制得具有"串珠"丝状结构的复合纤维膜,并观察微球及纤维膜的体外释药过程。结果:电纺技术制备出具有一定力学强度的缓释微球的纤维膜。体外释药在50hr时纤维膜释放速率78%,较微球的释放速率96%缓慢,之后各自接近平缓释放。结论:用静电纺丝技术平台制备缓释再生引导膜是可行的,这一技术为牙周组织再生术的完善和发展提供新的研究空间,其生物学性能尚需进一步研究。Objective：Based on fibers electrospinning technology,we prepared periodontal tissue regeneration membrane with microspheres from which bone morphogenetic protein growth factor （rhBMP2） are sustaining released.Methods：By mixing the calcium alginate microspheres carrierd of rhBMP-2 in with Carboxymethyl chitosan and CCS /nHA,and making use of electrospinning technique,we made fibericated membrane of beads-in-fiber structure.And then,we observed the medication releasing process from both microspheres and membrane.Results：Making use of this new method,a new periodontal tissue regeneration membrane with higher mechanical strength can be prepared.It is found that medication releasing growth factor released more slowly （with a rate of 78%） than which from microspheres （which rate is 96%）.This shows its postponed releasing effect.Conclusion：It is possible to prepare periodontal tissue regeneration membrane by using electrospinning technology.This study provided a new research space for periodontal tissue regeneration.However,its biological properties should be studied further.

关 键 词：静电纺丝 缓释 微球 生长因子(rhBMP-2) 羧甲基壳聚糖 羟基磷灰石(CCS/nHA) 引导牙周组织再生 

分 类 号：R944[医药卫生—药剂学]