DNA与荧光分子BCDO相互作用研究  

Study on the interaction of DNA with fluorescent BCDO

作　　者：刘小芳[1] 李平[1] 苗婷婷[1] 李栋[1] 倪翔[1] 林琳[1] 

机构地区：[1]天津理工大学化学化工学院,天津300384

出　　处：《天津理工大学学报》2010年第6期32-35,共4页Journal of Tianjin University of Technology

基　　金：国家自然科学基金(21071109)

摘　　要：近年来,在DNA检测中荧光光谱法被广泛应用.新型荧光探针化合物的发现成为当前研究的热点,本文以自主发现的荧光分子4H,4'H-2,2'-双色烯-4,4'二酮(简称BCDO)为荧光探针,采用荧光猝灭法,通过体系荧光光谱的测定,对其与小牛胸腺DNA的作用方式进行了研究.通过DNA溶液浓度对荧光分子BCDO的影响实验,F0/F～C曲线的绘制,发现BCDO与DNA的相互作用为静态猝灭过程,其猝灭常数Kq在106～108数量级之间,大于106数量级,说明两者之间有很强的亲和能力.由Na+对BCDO-DNA体系的影响实验进一步说明了BCDO与DNA之间的相互作用方式为静电作用.In recent years,fluorescence spectrometry was widely used in the test of DNA.The discovery of new fluorescent compounds was currently a hot research.In this paper,self-made 4H,4′H-2,2′-bichromene-4,4′-dione（referred to BCDO） was used as a fluorescent probe,the interaction of BDCO and DNA was investigated by fluorescence spectrometry.Through the experiment on the fluorescence quenching spectra of BCDO by DNA at different concentrations,drawing F0/F～C curve,it was found that the interaction of BCDO and DNA is a static quenching process,the quenching constant was in excess of 106,it was showed that BDCO with DNA have strong affinity .The Na＋ experimental results further indicated that BDCO interacted with DNA in electro static binding mode.

关 键 词：4H 4'H-2 2'-双色烯-4 4'二酮 小牛胸腺DNA 荧光猝灭法 相互作用 

分 类 号：O657.3[理学—分析化学]