高效液相色谱法测定氨甲苯酸对甲基苯甲酸和对羧基苯甲酸含量  

Content analyzing of paratoluic acid and terephthalic acid in p-aminomethyl benzoic acid by HPLC

作　　者：张嗣军 侯奇伟 

机构地区：[1]湖南怀化医专附属医院暨怀化市第三人民医院,湖南怀化418000 [2]湖南洞庭药业股份有限公司,湖南常德415001

出　　处：《湖南中医药大学学报》2010年第12期5-7,共3页Journal of Hunan University of Chinese Medicine

摘　　要：目的建立HPLC法同时测定氨甲苯酸中杂质对甲基苯甲酸和对羧基苯甲酸含量。方法采用高效液相色谱法[1],色谱柱C18柱,流动相,甲醇:乙腈:水:冰乙酸(25:15:60:1.5),检测波长为230nm。结果氨甲苯酸浓度在1.01～5.04μg/mL内与峰面积呈良好线性关系,最低检测限为0.86μg/mL。结论本法简便、准确,可用于控制氨甲苯酸原料药的质量。Objective to establish an HPLC method for analyzing the content of paratoluic acid and terephthalic acid in p-aminomethyl benzoic acid at the same time.Methods HPLC1,column is octadecylsilane bonded silica gel（ODS）, mobile phase was made from methanol：acetonitrile：water：glacial acetic acid（25：15：60：1.5）,detecting wave length is 230nm.Results the peak area shows fine linear relationship when concentration of p-aminomethyl benzoic acid within 1.01～5.04μg/mL,limit of detection is 0.86μg/mL.Conclusion this method is simple and exact,can be used in quality controlling of the API,p-aminomethyl benzoic acid.

关 键 词：氨甲苯酸 对甲基苯甲酸 对羧基苯甲酸 HPLC法 

分 类 号：R94[医药卫生—药剂学]