拟南芥18srRNA参与隐花色素介导的信号传导途径的研究  

A Study of 18srRNA Invovled in Cryptochrome-mediated Signaling in Arabidopsis

作　　者：汪启明[1,2] 屠小菊[1] 唐冬英[1] 赵小英[1] 刘选明[1] 

机构地区：[1]湖南大学生物学院化学生物传感与计量学国家重点实验室,湖南长沙410082 [2]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《湖南大学学报（自然科学版）》2010年第12期66-71,共6页Journal of Hunan University:Natural Sciences

基　　金：国家自然科学基金资助项目(30770200);教育部高校博士点基金资助项目(755228001)

摘　　要：通过筛选以拟南芥蓝光受体隐花色素双突变体cry1cry2为遗传背景的激活标签突变体库,得到一株SCC98-D株系,该突变体具有早开花,下胚轴变短,并具有花瓣数目增加等表型,彻底恢复了cry1cry2的晚开花和长下胚轴表型.通过Tail-PCR的方法克隆得到SCC98-D突变体中激活标签插入位点的侧翼序列,通过对该侧翼序列测序发现插入位点在18srRNA的1751bp处,运用RT-PCR方法分析插入位点周围基因的表达,发现只有18srRNA的表达被激活,由此证实了18srRNA参与隐花色素介导的信号传导途径.SCC98-D was screened from an activation-tagging mutant library in the background of blue light-receptor cryptochrome double mutant cry1cry2 in Arabidopsis,which was early flowering,short hypocotyl and excess petal number,and then the phenotype of late flowering and long hypocotyl in cry1cry2 was suppressed.The flanking sequence of inserted site was cloned with TAIL-PCR.The sequenced result of flanking sequence has shown that the T-DNA was inserted in 1751 bp of 18srRNA.Afterwards,a transcriptional analysis of the gene around inserted site with RT-PCR demonstrated that the activated gene was 18srRNA.This study has identified that 18srRNA is involved in Cry-mediated signaling.

关 键 词：拟南芥 隐花色素 激活标签突变体 18s核糖体RNA 

分 类 号：O613.52[理学—无机化学]