Influence of copper solvent extractant on microbial community structure of acidophilic microorganisms  

作　　者：陈勃伟 李文娟 刘兴宇 周桂英 温建康 

机构地区：[1]National Engineering Laboratory of Biohydrometallurgy,General Research Institute for Nonferrous Metals

出　　处：《Journal of Central South University》2010年第6期1196-1200,共5页中南大学学报（英文版）

基　　金：Project(50904011) supported by the National Natural Science Foundation of China

摘　　要：The influence of different concentrations of copper solvent extractant ZJ 988 on the growth and activity of acidophilic microorganisms was studied and the microbial community structures were compared by 16S rRNA gene clone library analysis.The total bacteria numbers are reduced when 0.5%(volume fraction) extractant is added.The proportions of Acidithiobacillus ferrooxidans and Acidiphilium organovorum are increased,whereas the proportion of Leptospirillum ferriphilum is reduced.When the concentration of extractant is elevated to 1%,growth of all bacteria is inhibited.Clone library results reveal that the dominant bacteria in the culture solution with/without the extractant are At.ferrooxidans,A.organovorum and L.ferriphilum.The sensitivity order of the three bacteria to the extractant from the most to the least is found to be L.ferriphilum>At.ferrooxidans>A.organovorum.The influence of different concentrations of copper solvent extractant ZJ 988 on the growth and activity of acidophilic microorganisms was studied and the microbial community structures were compared by 16S rRNA gene clone library analysis. The total bacteria numbers are reduced when 0.5% （volume fraction） extractant is added. The proportions of Acidithiobacillus ferrooxidans and Acidiphilium organovorum are increased, whereas the proportion of Leptospirillum ferriphilum is reduced. When the concentration of extractant is elevated to 1%, growth of all bacteria is inhibited. Clone library results reveal that the dominant bacteria in the culture solution with/without the extractant are At. ferrooxidans, A. organovorum and L. ferriphilum. The sensitivity order of the three bacteria to the extractant from the most to the least is found to be L. ferriphilum〉At, ferrooxidans〉A. organovorum.

关 键 词：EXTRACTANT ACIDOPHILE 16S rRNA gene BIOHYDROMETALLURGY 

分 类 号：Q938[生物学—微生物学]