夏天无总碱的精制工艺研究  

A Study on the Purification of Total Alkaloids from Xiatianwu Alkalods

作　　者：马宏达[1] 郭涛[1] 颜鸣[1] 程非[1] 朴希晨[2] 

机构地区：[1]沈阳军区总医院药剂科,辽宁沈阳110840 [2]大连医科大学

出　　处：《解放军药学学报》2010年第6期485-488,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：辽宁省博士科研启动基金;No.20101133

摘　　要：目的研究以阳离子交换树脂精制夏天无中总生物碱的方法及其工艺条件。方法采用紫外分光光度法测定夏天无总碱含量,高效液相色谱法测定原阿片碱,延胡索乙素含量。采用732型阳离子交换树脂精制夏天无中总生物碱,考察工艺和参数条件。结果阳离子树脂对夏天无总碱的最大吸附量为31.33 mg/g。上样浓度:1 g/ml。上样流速:1 ml/min,洗脱方法:先用蒸馏水除杂,经氨水碱化后,再用3 BV 80%乙醇洗脱,洗脱率为89.5%,得到的总生物碱含量为62.4%。再经酸碱沉淀纯化后的夏天无总生物碱的含量大于70%。结论阳离子交换树脂对夏天无总生物碱的吸附能力较好,可用于夏天无总生物碱的提取精制。Objective To study the methods and technological parameters of the purification process of total alkaloids from herb using cation exchange resin.Methods In order to select the optimal technological parameters,the content of total alkaloids was determined by spectrophotometry.The content of protopine and tetrahydropalmatine was determined by high performance liquid chromatography.Results One gram cation exchange resin could absorb 31.33 mg/g of the total alkaloids.The optimized condition for purification was as follows： the concentration of the sample solution was 1 g/ml,and the flow rate was 1 ml/min.80% alcohol was used as the eluent and the quantity of the eluent was about three times the bed volume 89.5% of the total alkaloids could be washed off.The total alkaloids exceeded 70% after acid-alkali precipitation.Conclusion Cation exchange resin can well absorb the total alkaloids from herb.It can be used to pureify total alkaloids from Corydalis Decumbentis Rhizoma.

关 键 词：阳离子交换树脂 夏天无 生物碱 原阿片碱 延胡索乙素 

分 类 号：R943[医药卫生—药剂学]