三氯乙烯致豚鼠皮肤变态反应和血清免疫球蛋白水平改变  

Skin allergic reaction and serum immunoglobulin changes in guinea pigs treated with trichloroethylene

作　　者：周丽[1] 徐新云[1] 李学余[1] 柯跃斌[1] 史才枫[1] 刘月峰[1] 

机构地区：[1]广东省深圳市疾病预防控制中心,广东深圳518020

出　　处：《中国卫生检验杂志》2010年第12期3098-3100,共3页Chinese Journal of Health Laboratory Technology

基　　金：深圳市科技计划项目资助课题(200802073)

摘　　要：目的:探讨三氯乙烯(TCE)对豚鼠皮肤变态反应和血清免疫球蛋白水平的改变。方法:采用豚鼠最大值试验GPMT,动物分成阴性对照组、阳性对照组和TCE实验组,每组6只豚鼠,分别皮内注射橄榄油、2,4-二硝基氯苯(DNCB)和TCE。实验结束后观察动物皮肤改变,应用自动生化分析仪检测致敏动物血清免疫球蛋白水平。结果:阳性对照组和TCE实验组动物出现明显皮肤损害,阳性对照组动物致敏率为100%,TCE实验组致敏率为83.3%。TCE致敏豚鼠血清总IgG含量明显高于阴性对照组(P<0.05)。结论:三氯乙烯属于强致敏物,可诱导豚鼠产生皮肤变态反应,引起血清免疫球蛋白改变。Objective：To study the skin allergic reaction and serum immunoglobulin changes in guinea pigs after treated with trichloroethylene（TCE）.Methods：Guinea pig maximization test（GPMT） was applied in this study,guinea pigs were divided into 3 groups,namely negative control,positive control and TCE treatment.the animals of 3 groups were administrated with olive oil,2,4-dinitrochlorobenzene（DNCB）,and TCE,respectively,by intradermal injection.The animal skin was observed and blood was collected after various treatment,the levels of serum immunoglobulin IgM,IgG,IgA,C3,C4 were detected with automatic biochemical analyzer.Results： Obvious skin impairment was observed in the groups of positive control and TCE treatment,the skin impairment included erythema and edema,the sensitization rate was 100% in positive control and 83.3% in TCE treatment group.Additionally,IgG levels elevated significantly in the groups of positive control and TCE treatment when compared with the negative control.Conclusion： Trichloroethylene is one of the strong hypersensitizing substances,it could induce skin allergic reaction and serum immunoglobulin changes in guinea pigs.

关 键 词：三氯乙烯 皮肤 变态反应 免疫球蛋白 豚鼠最大值试验 

分 类 号：R994.3[医药卫生—毒理学]