绍兴地区成年女性高危型人乳头瘤病毒感染率及年龄关系探讨  

Genotype prevalence and age distribution of high risk human papillomavirus (HPV) in Shaoxing

作　　者：余鑫煜[1] 郑专[1] 杨明峰[1] 

机构地区：[1]浙江省绍兴市人民医院分子医学中心,浙江绍兴312000

出　　处：《中国卫生检验杂志》2010年第12期3387-3388,3391,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:探讨绍兴地区成年女性高危型人乳头瘤病毒(HPV)的感染情况及其与年龄的关系,以探讨高危型HPV感染的临床意义。方法:以核酸分子快速杂交仪为平台,利用导流杂交技术同时检测15种高危亚型,用统计软件分析结果。结果:576例女性标本中,检测到高危型人乳头瘤病毒标本172例,阳性率为29.86%。高危亚型主要以HPV16、HPV52、HPV58、HPV31、HPV18等为主。阳性标本中,单一感染122例(70.93%),二重感染34例(19.78%),三重及以上感染16例(9.3%)。单一感染主要以HPV16、HPV58、HPV52为主;多重感染中以HPV52合并HPV16、58的感染最为多见。在年龄组中,筛查人群以21岁～40岁的妇女为主,41岁以后检测人数骤然减少。HPV感染高峰出现在21岁～25岁,检测阳性率远高于平均水平29.86%。结论:绍兴地区女性宫颈高危型HPV感染率较高,开展各型HPV检测对于HPV感染和子宫颈癌的诊断、治疗具有重要意义。Objective：To report the genotype prevalence and age distribution of high risk human papillomavirus（HPV） in Shaoxing women who were suspicious as HPV infection.Methods： A low density gene chip containing 15 high risk types was detected by flow through rapid hybridization after DNA amplified by PCR technology,then analyzed by EXCEL 2003 and SPSS 16.0.Results： 172 samples（29.86%） were HPV positive among 576 clinical specimens.HPV16,HPV52,HPV58,HPV31,and HPV18 were the most frequent high risk subtypes.Among 172 positive specimens,122 cases（70.93%%） were HPV single infection,34 cases（19.78%） double infection,and 16 cases（9.3%） more than double infection.Women with clinical detection of HPV infection were mainly between 21 to 40 years old.Most women were single infection even in different age group,but HPV infection trend was towards younger.Conclusion： The high risk HPV infection rate is high correspondingly in Shaoxing,and the determination of HPV genotypes was important for early discovery,prevention and treatment of cervical cancer.

关 键 词：高危型人乳头瘤病毒 聚合酶链反应 基因分型 

分 类 号：R711.32[医药卫生—妇产科学]