葡萄球菌肠毒素B诱导脐静脉内皮细胞凋亡机制的探讨  

Mechanisms of apoptosis in human umbilical vein endothelial cells by Staphylococcal enterotoxin B

作　　者：管俊昌[1] 刘婷婷[1] 朱琍燕[2] 马丽娜[1] 闵宏林[1] 李凤云[1] 

机构地区：[1]蚌埠医学院安徽省感染与免疫重点实验室病原生物学教研室,安徽蚌埠233030 [2]苏州大学基础医学与生物科学学院围产与胚胎学研究室,江苏苏州215123

出　　处：《中国微生态学杂志》2010年第12期1085-1088,共4页Chinese Journal of Microecology

基　　金：国家自然科学基金(81070506);安徽省教育厅自然科学研究计划项目(2006KJ393B)

摘　　要：目的探讨金黄色葡萄球菌(金葡菌)肠毒素B诱导脐静脉内皮细胞凋亡的机制。方法将不同浓度金葡菌肠毒素B感染脐静脉内皮细胞8 h后,用流式细胞术检测细胞凋亡率,同时用比色法检测TNF-α、caspase-3及caspase-8的产生量,并检测加入TNF-α抗体、caspase-3和caspase-8抑制剂后的细胞凋亡率。结果不同浓度肠毒素B作用脐静脉内皮细胞8 h后均可诱导细胞凋亡,且TNF-α、caspase-3和caspase-8的产生量均高于对照组(P<0.01);而加入TNF-α抗体、caspase-3和caspase-8抑制剂后凋亡率明显降低。结论金葡菌肠毒素B可以诱导脐静脉内皮细胞凋亡,其凋亡机制可能是通过TNF-α介导的caspase-8及caspase-3激活的外源性死亡因子受体途径。Objective To study the mechanisms of apoptosis in human umbilical vein endothelial cells induced by Staphylococcal entertoxin B（SEB）.Method For 8 hours after human umbilical vein endothelial cells（HUVEC） were infected by SEB,the apoptosis rate of HUVEC was determined by flow cytometry,and the productions of TNF-α,caspase 3 and caspase 8 in the HUVEC were detected by colorimetric assay.Meanwhile,SEB-induced apoptosis was also determined by flow cytometry after the antibody against TNF-α,inhibitor of caspase 3 or caspase 8 was added,prior to SEB respectively in the HUVEC.Result SEB significantly induced the apoptosis of HUVEC,accompanied with the significant increase in the production of TNF-α,caspase 3 and caspase 8.But the apoptosis rates of HUVEC were significantly inhibited by antibody against TNF-α,inhibitor of caspase 3 or caspase 8,respectively.Conclusion SEB is a mediator of staphylococcus aureus inducing the apoptosis of HUVEC and can activate caspases via the extrinsic death pathway mediated by TNF-α.

关 键 词：金黄色葡萄球菌 肠毒素B 脐静脉内皮细胞 凋亡 

分 类 号：R378.11[医药卫生—病原生物学]