导入额外拷贝nifA基因的大豆根瘤菌工程菌株的构建  

Construction of genetic engineering strain of introduction extra nifA gene into native Rhizobium japonicum

作　　者：李海英[1] 张喜波[1] 刘金玲[1] 于海鹏[1] 杨乐[1] 蒙明明[1] 贾珊珊[1] 

机构地区：[1]黑龙江大学生命科学学院黑龙江省普通高等学校分子生物学重点实验室,哈尔滨150080

出　　处：《黑龙江大学自然科学学报》2010年第6期744-746,共3页Journal of Natural Science of Heilongjiang University

基　　金：黑龙江省科技攻关项目(GA08B101);哈尔滨市科技创新人才研究专项资金项目(RC2009XK001004)

摘　　要：以快生型大豆根瘤菌(Sinorhizobium fredii)15067的基因组DNA为模板,采用PCR技术,克隆固氮基因nifA全长;将其连入带有lac启动子下游,构建带有LuxAB基因标记的表达载体pTR102,采用三亲本杂交技术转化土著大豆根瘤菌中,构建大豆根瘤菌工程菌株。为研究转基因工程菌株对大豆固氮能力的影响奠定基础。PCR technology was used to clone the full length of nodulation gene nifA by using template of genomic DNA from Sinorhizobium fredii 15067 in this study.The expression vectors of Prokaryotae consist of lac promoter was constructed by using plasmid pTR102 marked by luxAB gene.The expression vector were transformed into native Rhizobium japonicum with method of triparental hybridization to construct genetic engineering strain.It will lay a foundation for the study of genetic engineering strain influence on soybean nitrogen-fixing nodulation capacity.

关 键 词：大豆根瘤菌 NIFA基因 三亲本杂交 大豆根瘤菌工程菌株 

分 类 号：S566.3[农业科学—作物学] S188