hNIS基因转染介导碘-131杀伤肝癌细胞的研究  

Research of Kill the Liver-cancer Cells by Transfect hNIS Gene into It Acrosse Radioactive Iodine Uptake

作　　者：朱伟[1] 宁尚义[1] 李素萍 孙作忠[1] 王传玺[3] 于文霞[1] 孙自平[1] 孟斌[1] 

机构地区：[1]山东省医学科学院放射医学研究所,山东济南250062 [2]山东省鄄城县闫什计生站 [3]山东大学附属省立医院肿瘤治疗中心

出　　处：《中国辐射卫生》2010年第4期391-393,共3页Chinese Journal of Radiological Health

基　　金：山东省自然基金青年基金项目(Q2007C07)

摘　　要：目的将hNIS基因转染到肝癌细胞使其具备主动吸收碘的功能,观察转基因肝癌细胞吸收碘的能力,并进一步观察其介导放射性碘杀伤肝癌细胞的可能性。方法重组含有hNIS的载体,并转染至肝癌细胞HepG2,利用稳定表达的转基因肝癌细胞进行吸碘能力测定,并应用MTT法测定碘-131杀伤肝癌细胞的效果。结果γ计数仪测定表达hNIS基因的肝癌细胞吸收碘的能力比对照组高10倍,MTT法测定结果显示表达hNIS基因的肝癌细胞抑制率明显高于对照组。结论转基因肝癌细胞具有浓聚碘的能力,并且可以介导碘-131杀伤肝癌细胞。Objective We transfected the human sodium / iodide symporter（hNIS） gene into liver-cancer cells to help it have the ability of iodine uptake to assess the effect of radioactive iodine uptake by liver-cancer cells and the role of anti-hepatoma by radioactive iodine.Methods Recombinant pCDNA-hNIS was transfected into HepG2 cells.The level of 131Ⅰuptake was assessed using transgenic liver cells stably expressing hNIS and effect of anti-hepatoma was determined by MTT method.Results 10-fold 131Ⅰuptake was observed in HepG2 cells transfected with pCDNA-hNIS compared with the control group by γ counting,correspondingly.The effect of anti-hepatoma was significantly higher in transgenic HepG2 cells compared with the controls.Conclusion HepG2 cells transfected with pCDNA-hNIS could uptake 131Ⅰ,accordingly,inhibited by 131Ⅰ.

关 键 词：人钠/碘同向转运体 肝癌 基因转染 放射性碘治疗 

分 类 号：Q691[生物学—生物物理学]