血府逐瘀汤对大鼠心肌缺血再灌注损伤的保护作用  

Protective effects of Xuefu Zhuyu decoction on myocardium ischemia reperfusion injury in rats

作　　者：唐丹丽[1] 刘寨华[2] 张华敏[3] 孙明杰[1] 隋宇[1] 

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]中国中医科学院中医基础理论研究所,北京100700 [3]中国中医科学院中医药信息研究所,北京100700

出　　处：《中国中药杂志》2010年第22期3077-3079,共3页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30701066)

摘　　要：目的:观察血府逐瘀汤对心肌缺血再灌注损伤大鼠的保护作用。方法:36只雄性SD大鼠随机分为假手术组、模型组及血府逐瘀汤组。可逆性冠脉左前降支结扎缺血30 min再灌注2 h复制MI/RI模型,检测再灌注后各组大鼠血清CK,LDH-L以及IL-1β,IL-6,IL-10含量,并在电镜下观察心肌超微结构变化。结果:与模型组比较,血府逐瘀汤组能降低血清IL-1β,IL-6水平,促进IL-10的表达(P<0.01),同时减轻心肌超微结构的损伤;血清CK及LDH-L含量有所下降,但与模型组之间差异不显著。结论:血府逐瘀汤对MI/RI大鼠有保护作用,与调控炎症反应并保护心肌超微结构有关。Objective： To investigate the protective effects of Xuefu Zhuyu decoction on myocardium ischemia reperfusion injury in rats.Method： 36 SD rats were divided into 3 groups randomly,sham-operated group,model group,Xuefu Zhuyu decoction group.The model of MI/RI of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats.Serum contents of LDH-L,CK were measured,the levels of serum IL-1β,IL-6 and IL-10 were measured and myocardial ultrastructure at the ischemia region was observed under the transmission electron microscope after myocardial reperfusion injury.Result： Compared with model group,IL-1β,IL-6 and IL-10 levels were lower,myocardial ultrastructural changes were improved in Xuefu Zhuyu decoction group（P〈0.01）,however,serum contents of LDH-L and CK no significant difference were found among the model group and Xuefu Zhuyu decoction group.Conclusion： Xuefu Zhuyu decoction can protect myocardium from MI/RI,the mechanism of action was related to inhibiting inflammatory reaction and reducing arrhythmia and the injury of the myocardial ultrastructure.

关 键 词：血府逐瘀汤 心肌缺血再灌注损伤 心肌酶 炎症因子 

分 类 号：R285.5[医药卫生—中药学]