利用RAPD技术对不同地理环境下柳松菇菌株亲缘关系的分析  

GENETICDIVERSITYANALYSIS OF DIFFERENTISOLATES OF Agrocybeaegerita USINGRANDOM AMPLIFIED POLYMORPHICDNA(RAPD) MARKERS

作　　者：谭琦[1] 严培兰[1] 詹才新[1] 郭力刚[1] 王南[1] 贺冬梅[1] 凌霞芬[1] 陈明杰[1] 潘迎捷[1] 

机构地区：[1]上海市农业科学院食用菌研究所

出　　处：《上海农业学报》1999年第4期18-21,共4页Acta Agriculturae Shanghai

基　　金：.NULL.

摘　　要：选用１４ 个柳松菇野生菌株（其中，来自中国６ 株，源于希腊６株，源于法国、印度各１株），利用ＲＡＰＤ 技术进行遗传差异测定。２０ 个随机引物中有１１ 个引物共扩增出９６ 条ＤＮＡ 条带，在此基础上分析并构建了遗传相关聚类图。结果表明，希腊的６ 个菌株遗传差异较小，它们之间的相似系数＞ ９０％ ，与法国的菌株亲缘关系也较近，相似系数在７０％ 左右。我国６ 个野生种菌株之间的遗传差异较大，相似系数为３０％ ～８５％ ，而与国外菌株的遗传相似性仅２０％ 强。In this research,RAPD m arkers were used to survey genetic variability among 14 isolates ofAgrocybe aegerita,which collected from differentcountries (6 isolates from China,6 from Greece,1 from France and 1 from India.).96 scorable DNA products were detected by 11 primers am ong the 20 tested prim ers.All of these products were analyzed to measureisolaterelationshipsby unweighted pairgroup m ethod using arithm etic. The UPGMAillustrated that6isolatesfrom Greecehad ahighergeneticrelativity with m ore than 90% sim ilarity indexes among them and showed a relationship w ith an about 70% sim ilarity to the isolate from France.A.aegerita germ plasms from China showed a higher geneticdiversity.Thegeneticsim ilarity indexesofthe6 isolatesfrom China varied from 30% to 85% .Thesimilarity ofthe 6 strainsto theisolates from foreign countrieswasonly 20% .

关 键 词：柳松菇 菌株 RAPD 遗传分析 

分 类 号：S646.1[农业科学—蔬菜学]