兔出血症病毒结构多肽的研究  

STUDIES ON STRUCTURAL POLYPEPTIDES OF RABBIT HAEMORRHAGIC DISEASE VIRUS(RHDV)

作　　者：王恒安[1] 杜念兴[2] 徐为燕[2] 

机构地区：[1]上海农学院动物科学系,上海201101 [2]南京农业大学,南京210095

出　　处：《上海农业学报》1999年第4期56-59,共4页Acta Agriculturae Shanghai

摘　　要：纯化ＲＨＤＶ 经ＳＤＳＰＡＧＥ、硝酸银染色显示８ 条多肽，分子量分别为８５．１，７１．２，６４．５，６１．４，５７．３，５４．０，２９．２，２８．１ ｋＤ，免疫转印结果确认其中的６ 条多肽为病毒结构多肽，分子量分别为６４．５，６１．４，５７．３，５４．０，２９．２，２８．１ ｋＤ，且其相对百分含量分别为６５．８７，３．１７，３．９７，１０．３２，９．５２，７．１４％ ，表明分子量为６４．５ ｋＤ的多肽为病毒主要结构多肽。Eight polypeptides of the purified RHDV,w ith m olecular w eight of 85.1, 71.2,64.5,61.4,57.3,54.0,29.2 and 28.1 kD,w ere detected by SDS PAGE stained w ith silver. By im m unoblotting analysis, six of them w ere determ ined to be viral structural polypeptides w ith theirrespective m olecularw eightsof64.5,61.4,57.3,54.0,29.2 and 28.1 kD,and the relative quantities of the six polypeptides w ere 65.87% , 3. 17% , 3. 97% , 10.32% ,9.52% and 7.14% ,respectively.This resultshow ed thatthe polypeptide of64.5 kD w as the m ain structuralpolypeptide ofRHDV.

关 键 词：兔出血症病毒 结构多肽 免疫转印 

分 类 号：S852.65[农业科学—基础兽医学]