紫花苜蓿对三叶草核盘菌敏感性差异的生理生化特性研究  

Physiological and Biological Indexes in Alfalfa Leaves as Influenced by Sclerotinia trifoliorum Inoculation

作　　者：倪郁[1] 尹亚丽[2] 郭彦军[2] 韩龙[2] 唐华[2] 

机构地区：[1]西南大学农学与生物科技学院,重庆400715 [2]西南大学动物科技学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2010年第12期19-24,共6页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30800802)

摘　　要：在田间收集发病苜蓿菌核,经PDA培养基再生培养,紫花苜蓿分枝期将菌核分别接种到"三得利"、"WL324"、"阿尔刚金"和"FGC901"品种上,研究接种三叶草核盘菌对紫花苜蓿叶片相关生理生化指标的影响.结果表明:品种"三得利"与"WL324"抗性相对较高,"阿尔刚金"与"FGC901"抗性相对较低;抗性品种"三得利"SOD酶活性早期增加,后期下降,感性品种"FGC901"变化趋势相反;接种后抗性品种POD酶活性与感性品种相比呈下降趋势,CAT酶活性整体高于感性品种,而PAL酶活性无规律性变化;抗性品种后期MDA、脯氨酸含量增幅减少,可溶性蛋白含量下降幅度降低.The isolates of Sclerotinia sclerotiorum from field host-plants were cultured in the PDA culture medium.Then mycelium from the culture was inoculated around plant stems of four alfalfa varieties in pot using a gauze during the branching period of the plants to study the effects of inoculating S.trifoliorumin on the physiological and biochemical indexes of the leaves.The results showed that varieties Sanditi and WL324 had higher resistance to S.trifoliorum than FGC901 and Algonguin.The SOD activity in the resistant variety Sanditi increased first and then decreased after inoculation,while that in the sensitive variety FGC901 decreased first and then increased.After inoculation,the POD activity of sensitive alfalfa increased and that of resistant alfalfa decreased.The activity of CAT in resistant alfalfa was higher than that in sensitive alfalfa.No regular change was found for PAL activity.The content of malondialdehyde （MDA） and soluble proline in all alfalfa varieties increased first after inoculation,while the increment rate of MDA decreased in resistant alfalfa and increased in sensitive alfalfa.The content of soluble protein （SP） in alfalfa leaves decreased after inoculation,while the decreasing rate dropped in resistant alfalfa and increased in sensitive alfalfa.

关 键 词：三叶草核盘菌 紫花苜蓿 生理生化指标 

分 类 号：S541[农业科学—作物学]