罗布麻种质的过氧化物酶及酯酶同工酶研究  

Research of Peroxidase Isozyme and Esterase Isozyme of Germplasms of Apocynum venetum Linn.

作　　者：任晓月[1] 陈彦云[1,2] 

机构地区：[1]宁夏大学西北退化生态系统恢复与重建教育部重点实验室,宁夏银川750021 [2]宁夏大学生命科学学院,宁夏银川750021

出　　处：《北方园艺》2010年第24期134-137,共4页Northern Horticulture

基　　金：宁夏自然科学基金资助项目(NZ0807)

摘　　要：采用聚丙烯酰胺凝胶垂直板电泳技术,对不同地区罗布麻过氧化物酶和酯酶同工酶酶谱进行分析。结果表明:过氧化物酶具有2～5条不等的酶带,Rf值在0.547～0.882之间;酯酶具有3～5条不等的酶带,Rf值范围在0.1769～0.5918之间;过氧化物酶和酯酶的酶带数、酶带迁移率和酶活性可反映不同地区罗布麻种群间和种群内存在很大的遗传差异;DPS聚类分析结果表明:新疆红麻与内蒙红麻亲缘关系最近,宁夏红麻与河北红麻亲缘关系较近,青海白麻与内蒙古、新疆、宁夏和河北地区的亲缘关系最远。The zymograms of peroxidase isozyme（POD） and esterase isozyme（EST） of Apocynum venetum Linn.from different areas were analyzed with polyacrylemide gel electrophoresis.The results showed that there were 2～5 different bands in the zymogram of peroxidase isozyme（POD）,with Rf values between 0.547 and 0.882 and 3～5 different bands in the zymogram of esterase isozyme（EST） with Rf values between 0.1769 and 0.5918.The bands,Rf values and activities of POD and EST of Apocynum venetum Linn.from different areas illustrated that there were great inheritance differences between and in the populations.DPS cluster analysis indicated that the variety of Apocynum venetum L.from Xinjiang had closest genetic relationship with the variety from Inner Mongolia,and the variety from Ningxia had closer genetic relationships with the variety from Hebei.The variety of Poacynum hendersonii（Hook.f） Woodson from Qinghai had the farthest genetic relationship with the other 4 varieties of Apocynum venetum L.from Xinjiang,Inner Mongolia,Ningxia and Hebei.

关 键 词：罗布麻 过氧化物同工酶 酯酶同工酶 亲缘关系 

分 类 号：S563.7[农业科学—作物学]