具有蛋白酶体β7亚基功能的棉铃虫HaProβ7基因的克隆与序列结构及表达研究  

Cloning,Sequence Structure and Expression of HaProβ7 Gene with Proteasome β7 Subunit Function in the Helicoverpa armigera

作　　者：王更先[1,2] 司马杨虎[1] 张升祥[3] 徐世清[1] 

机构地区：[1]苏州大学现代丝绸国家工程实验室,苏州大学基础医学与生物科学学院,江苏苏州215123 [2]邯郸学院生物科学系,河北邯郸056005 [3]山东农业大学林学院,山东泰安271018

出　　处：《北方园艺》2010年第24期138-142,共5页Northern Horticulture

基　　金：国家重点基础研究发展“973”计划资助项目(2005CB121005)

摘　　要：以棉铃虫中肠总RNA为模板,利用快速扩增cDNA末端(RACE)技术克隆了棉铃虫蛋白酶体β7亚基基因的cDNA序列,并对所得基因进行了序列分析。结果表明:棉铃虫蛋白酶体β7亚基基因的cDNA序列亚基全长1 007 bp,命名为HaProβ7(GeneBank登陆号:FJ378902),包含1个846 bp的完整开放读码框(ORF)序列,编码蛋白质为281个氨基酸残基,预测分子量30.07 kD,等电点为8.04。HaProβ7蛋白质在40～229氨基酸残基位置为蛋白酶体β7亚基的保守区域。Clustal W进行多序列比对发现,HaProβ7编码蛋白质与果蝇等昆虫蛋白酶体β7具有63%以上的同源性,蛋白酶体β7的保守区域高度一致。邻近(NJ)法分子进化分析也显示,HaProβ7编码蛋白质与其它生物蛋白酶体β7进化上同源。基因表达谱分析结果表明,Haproβ7在体壁、中肠和生殖腺中表达量较高,在头部表达量最少。By using total RNA which was extracted from midgut of Helicoverpa armigera and rapid amplication of cDNA ends（RACE） technology,the full-length cDNA encoling Proteasome β7 Subunit was cloned,then futher analyzed the gene sequence.The results showed that the full-length cDNA sequence was named as HaProβ7（GeneBank accession number：FJ378902）.It was 1 007 bp nucleotide long,contained an ORF（846 bp） and encoded 281 amino acids,with their predicted mass 30.07 KD and isoelectric point 8.04.The deduced amino acid showed that a proteasome β7 subunit domain between 40 to 229 amino-acid residue.It had more than 63% identity to other insects such as Drosophila melanogaster.The proteasome β7 subunit conservative regions were very similar with each other.Molecular evolution by Neighbor Joining method indicated that HaProβ7 had homologous with other proteasome β7 subunit of species.Sequence alignment analysis showed that the cloned gene was proteasome β7 subunit gene.The results of gene expression profiling analysis showed that Haproβ7 had high expression in body wall,midgut,gonad,but low expression in head.

关 键 词：棉铃虫 蛋白酶体 克隆 HaProβ7基因 RACE 

分 类 号：S435.622.3[农业科学—农业昆虫与害虫防治]