龙骨花的组织培养研究  

Study on Tissue Culture of Euphorbia trigona H.

作　　者：纪春艳[1] 张洋洋[1] 王颖[1] 

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157012

出　　处：《北方园艺》2010年第24期152-153,共2页Northern Horticulture

基　　金：黑龙江省教育厅科技基金面上资助项目(11551516)

摘　　要：在培养条件完全相同的条件下,对龙骨花进行组织离体培养。结果表明:带芽眼茎段和茎尖比叶片诱导效果好;以不同大小带芽眼茎段和茎尖作为外植体诱导愈伤组织及分化芽时,0.5 cm×0.5 cm较0.3 cm×0.3 cm大小的外植体效果好;选取0.5 cm×0.5 cm大小的茎段作为外植体进行组织培养,进行愈伤组织及芽诱导时用含6-BA1.0 mg/L+NAA0.5 mg/L的MS培养基效果最佳,进行生根诱导时以1/2MS附加NAA0.2 mg/L效果最佳。In the same culture conditions to study on the tissue culture in vitro of Euphorbia trigona H..The results showed that of eye with stem segment and shoot tip induced effect better than the leaf,using different sizes shoot tip and bud eye with stem segment as the explants was inducted callus and differentiation bud,the 0.5 cm×0.5 cm than 0.3 cm×0.3 cm size explants good effect,using 0.5 cm × 0.5 cm size of stem segment as explants,the optimal medium for callus and shoot induction was 6-BA 1.0 mg/L＋NAA 0.5 mg/L,the optimal medium for take root was 1/2 MS addited NAA 0.2 mg/L.

关 键 词：龙骨花 茎段 组织培养 

分 类 号：S681.903.6[农业科学—观赏园艺]