荠菜快繁体系的初步研究  

Preliminary Study on Rapid Propagation System of Capsella bursa-pastoris

作　　者：贾毛毛[1] 李学强[1] 李文静[1] 

机构地区：[1]河南科技大学林学院,河南洛阳471003

出　　处：《北方园艺》2010年第24期157-159,共3页Northern Horticulture

基　　金：河南科技大学大学生研究训练计划资助项目(SRTP2009113)

摘　　要：以荠菜种子为试材,研究种子消毒对接种污染率、冷处理对种子萌发率、初代和继代增殖培养基对丛生芽诱导以及生根培养基对丛生苗生根的影响,并以此为基础建立了无菌快繁体系。结果表明:1%NaClO消毒9 min最好,污染率为0,在4℃的冰箱中冷处理96 h萌发率较高,达36.97%。荠菜最适初代培养基为处理9,增殖系数达3.2,平均苗高达3.3 cm;最适继代培养基为处理3,增殖系数达2.27,最适生根培养基为处理3,平均根数4～6条,根较粗壮,生根率达73.08%。适宜的荠菜快繁体系为:新采集种子用1%NaClO消毒9 min,然后接种于MS培养基上在4℃的冰箱中冷处理96 h,将培养出的无菌苗去掉下胚轴后接种在MS+6-BA 2.0 mg/L+NAA 0.20 mg/L培养基上进行初代培养,在MS+6-BA 1.5 mg/L+NAA 0.12 mg/L培养基上进行增值培养,之后在MS培养基上进行壮苗培养,在MS+0.075 mg/LNAA培养基上进行生根培养,最后练苗移栽。Using Capsella bursa-pastoris seed as test material,the effect of seed disinfection on the rate of pollution,low-temperature treatment on seed germination,different culture media on primary and proliferation,different medium for rooting were studied.The results showed that the best disinfectant and sterilizing time were 1% NaClO and 9 minutes in that solution;Being treated for 96 h at 4℃,the germination rate could reach to 36.97%;The optimal medium for primary culture was the ninth treatment,the propagation coefficient was 3.2 and the average seedling height was 3.3 cm,the optimal medium for proliferation was the third treatment,the propagation coefficient was 2.27,the optimal medium for rooting was the third treatment,the number of healthy root was 4～6 and the inducing ratio of root was 73.08%.So the best rapid propagation system of Capsella bursa-pastoris was that,seed was treated in 1% NaClO for 9 minutes,being treated for 96 h at 4℃,the optimal medium for primary culture was MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L,the optimal medium for proliferation was MS＋6-BA 1.5 mg/L＋NAA 0.12 mg/L,the optimal medium for rooting was MS＋0.075 mg/L NAA.Then trained the seedlings and transplanted it finally.

关 键 词：荠菜 快速繁殖 试管培养 

分 类 号：S636.9[农业科学—蔬菜学]