α-LA干预对INS-1细胞氧化应激的影响  

Effect of α-lipoic acid on oxidative stress of INS-1 cells

作　　者：陈莹晖[1] 李裕明[2] 林松挺[3] 

机构地区：[1]海南医学院附属医院重症医学科,海南海口570102 [2]华中科技大学同济医学院附属协和医院,湖北武汉430022 [3]海口市人民医院,海南海口570208

出　　处：《海南医学院学报》2010年第12期1558-1561,1580,共5页Journal of Hainan Medical University

基　　金：海南医学院科研基金资助学报项目(0020100426)~~

摘　　要：目的:观察α-硫辛酸干预对INS-1细胞的作用及相关机制。方法:先用α-硫辛酸(200μmol/L)加入INS-1细胞中一起培养6 d,再应用不同浓度的葡萄糖作用于INS-1细胞48 h,抽取上清液测定MDA含量,并检测GSIS值,同时应用RT-PCR的方法检测UCP 2基因的表达,并与不加α-硫辛酸组互相比较。结果:(1)在相同糖浓度下,LA干预组与未加LA干预组相比,MDA含量升高,GSIS值降低,各浓度组相比均具有显著性差异;(2)在相同糖浓度下,-αLA干预组与未加-αLA干预组相比,UCP2mRNA表达增强且差异均有显著性。结论:-αLA为一个促氧化剂,能引起MDA含量增加,UCP2mRNA表达增强,GSIS值下降,说明-αLA在较长时间作用于胰岛细胞时起到促氧化的作用。Objective：To explore the effect of α-lipoic acid on INS-1 cells and the related mechanisms.Methods：α-lipoic acid 200 μmol/L was added into INS-1 cells for 6 days,glucose at different concentrations were applied in INS-1 cells for 48 hours.MDA were detected by spectrophotometer,then insulin secretion was measured by ELESA with high-glucose stimulation and UCP-2 gene expression was detected by RT-PCR.And all indexes were compared with these of none α-lipoic acid intervention group.Results：1 With the same glucose concentration,MDA in INS-1 cells with α-LA intervention was higher than that without intervention,and the GSIS value was reduced with significant differences among groups.UCP2 mRNA expression in intervention group increased,and there were significant differences among different groups.Conclusions：α-LA,as a promoting oxidants,can promot the expression of UCP2 mRNA and decline the glucose-stimulated insulin secretion,while increased MDA in INS-1 cells shows that α-LA can promote oxidation when affecting the islet cells during a long period of time.

关 键 词：UCP2 促氧化 氧化应激 GSIS -αLA 

分 类 号：R34[医药卫生—基础医学]