长白柳种质试管保存研究  

Study on the Germplasm Conservation in vitro of Salix polyadenia var. tschangbaischanica (Y.L.Chou et Chang) Y.L.Chou

作　　者：王德礼[1] 顾地周[2] 

机构地区：[1]通化市公园管理处,吉林通化134001 [2]通化师范学院生物系,吉林通化134002

出　　处：《安徽农业科学》2010年第34期19266-19267,共2页Journal of Anhui Agricultural Sciences

基　　金：国家科技部"国家科技攻关计划引导项目(2005BA741C)

摘　　要：[目的]筛选出长白柳嫩茎离体培养各阶段的培养基。[方法]以长白柳嫩茎基部直接再生形成的丛生芽团为试材,利用"延缓生长"的方法在试管内对长白柳进行种质保存,应用均匀设计法探讨了影响长白柳丛生芽团试管保存的主要因子和水平,筛选最适于长白柳丛生芽团试管保存的培养基。[结果]长白柳最适宜的丛生芽团试管保存培养基为:N-68+根皮苷3.20 mg/L+KT0.70 mg/L,保存43个月生长率仅为0.97%。对保存前后的芽苗生根情况对比结果表明,保存后的芽苗生根速度快,生根率达99.6%以上,较未保存前的生根率99.0%高。[结论]在试管内采取"延缓生长"法保存长白柳丛生芽团是切实可行的新方法。[Objective] The appropriate medium for Salix polyadenia var.tschangbaischanica（Y.L.Chou et Chang） Y.L.Chou culture in vitro was screened.[Method] The adventitious bud directly induced from the base of the tender shoot of Salix polyadenia var.tschangbaischanica（Y.L.Chou et Chang） Y.L.Chou was used as the explants and the germplasm conservation of the species was conducted by means of the method of deferring growth with its culturing in glass tube.The main factors with different levels for the germplasm conservation in vitro were investigated with the uniform design experiment for the screen of the best medium.[Result] The best suitable medium for in vitro conservation of the adventitious bud of the species was N-68＋3.20 mg/L of phloridzin＋0.70 mg/L of KT,under which the regeneration rate of it conserved for 43 months was 0.97%.The rooting rate of the adventitious bud treated by conservation in vitro was more than 99.6% with the quickly rooting ability.However,its rooting rate of non-treatment was only 99.0%.[Conclusion] It was proved that the new method of the conservation in vitro of the adventitious bud of Salix polyadenia var.tschangbaischanica（Y.L.Chou et Chang） Y.L.Chou was feasible.

关 键 词：长白柳 延缓生长 试管保存 根皮苷 均匀设计 

分 类 号：S792.12[农业科学—林木遗传育种]