哮喘儿童吸入丙酸氟替卡松后外周血单个核细胞Foxp3和磷酸化STAT5的变化(英文)  

Fluticasone increased Foxp3 in childhood asthma with upregulation of phosphorylated STAT5

作　　者：何云[1] 谢晓虹[2] 邓昱[2] 李欣 符州[2] 杨锡强[4] 刘恩梅[2] 

机构地区：[1]川北医学院附属医院儿科 [2]重庆医科大学附属儿童医院呼吸中心 [3]重庆医院大学附属儿童医院儿科研究所中心实验室 [4]重庆医科大学附属儿童医院肾脏免疫科

出　　处：《临床儿科杂志》2011年第1期1-7,共7页Journal of Clinical Pediatrics

基　　金：funded by Key Programme of Chongqing Science and Technology Commision(No.CSTC2007BA5013);National Nature Science Foundation of China(No.30470760);Program for New Century Excellent Talents in University(No.NCET-06-0775)

摘　　要：目的研究哮喘患儿吸入丙酸氟替卡松后外周血CD4+细胞的Foxp3的表达和变化,从而探讨儿童哮喘发生的可能机制。方法以30例确诊哮喘患儿为研究对象,随机分为未治疗哮喘组(15例)、吸入丙酸氟替卡松缓解组(15例),同期16例正常儿童为对照组。流式细胞仪检测外周血单个核细胞中的CD4+Foxp3+细胞比率,ELISA检测血浆和植物血凝素(PHA)体外刺激T细胞后上清液的IL-2、IL-6水平,Western blot检测外周血单个核细胞中磷酸化STAT5和非磷酸化STAT5的水平。结果哮喘组儿童外周血单个核细胞的CD4+Foxp3+细胞百分率低于正常对照组。与哮喘组相比,吸入丙酸氟替卡松缓解组儿童的CD4+Foxp3+细胞百分比有明显增高,血清IL-6水平降低而外周血单个核细胞磷酸化STAT5表达上调。结论哮喘儿童的外周血CD4+细胞的Foxp3表达下调,吸入激素可能通过上调磷酸化STAT5表达,诱导T细胞Foxp3表达,平衡T细胞应答,从而治疗儿童哮喘。Objective To evaluate the effects of fluticasone propionate（FP）on Foxp3 expression in CD4＋T cells,to explore the possible mechanisms of childhood asthma.Methods Thirty asthmatic children,15 with inhaled FP and 15 without inhaled FP,and 16 healthy children were recruited.Peripheral blood mononuclear cells（PBMC）labeled for CD4 and intracellular Foxp3 were analyzed using flow cytometry.The levels of IL-2 and IL-6 in serum and supernatant before and after stimulation by Phytohemagglutination（PHA）were measured by ELISA.The expression of phosphorylated signal transducer and activator of transcription 5（STAT5）in PBMC was detected by Western-blot.Results Compared with healthy control,the percentage of CD4＋ Foxp3＋ cells in PBMC in asthmatic children without inhaled FP was significantly decreased.After inhaled FP and in remission stage,the percentage of CD4＋ Foxp3＋ cells in asthmatic children was up regulated with a decreased serum IL-6 level and an increased phosphorylated STAT5 expression.Conclusions Decreased Foxp3 protein expression in peripheral CD4＋ T regulatory cells（Treg）is characterized in childhood asthma.Inhaled glucocorticoid therapy of childhood asthma might be attributed to its ability of increasing Foxp3 expression by upregulation of phosphorylated STAT5 to balance the T cell response.

关 键 词：哮喘 FOXP3 调节性T细胞 磷酸化STAT5 外周血单个核细胞 儿童 

分 类 号：R725.6[医药卫生—儿科]