三种人工合成寡核苷酸序列对体外培养的毛囊黑素细胞的影响  

Effects of three synthetie oligonucleotide sequence to melanocytes from human hair follicle

作　　者：李洪州[1,2] 薛东章[3] 张汝芝[1] 

机构地区：[1]蚌埠医学院第一附属医院皮肤性病科,安徽蚌埠233004 [2]安徽省蚌埠医学院药学系,安徽蚌埠233003 [3]解放军第123医院

出　　处：《中国美容医学》2011年第1期65-67,共3页Chinese Journal of Aesthetic Medicine

基　　金：安徽省第四批优秀青年科技基金(8040106819);2010中华医学会-欧莱雅中国人健康皮肤研究项目(S2010070833)

摘　　要：目的:研究三种人工合成寡核苷酸序列对体外培养的毛囊黑素细胞形态及酪氨酸酶活性的影响。方法:采用胶原酶和胰酶两步消化法,分离培养毛囊内黑素细胞。多巴氧化法测酪氨酸酶活性,硝酸银染色观察细胞形态的变化。结果:三种人工合成寡核苷酸序列可以显著增强酪氨酸酶活性(P<0.05);银染色细胞显示CpG-1826和CpG-2006组胞体变得肥大,黑素化明显,树突增多,与对照组相比有显著差异。结论:部分人工合成寡核苷酸序列可促进毛囊黑素细胞的酪氨酸酶活性和树突增加,为白癜风皮损复色的研究奠定基础。Objective To investigate effects of three synthetic oligonucleotides on tyrosinase activity and morphology of the melanocytes from fetal scalp hair follicles.Methods The follicle melanocytes were isolated and cultured by two steps digestion of collagenase and trypsin.Tyrosinase activity was measured by Dopa oxidation.Cell morphology was detected by silver nitrate staining.Results Melanocyte tyrosinase activity was promoted significantly by three synthetic oligonucleotides（P0.05）.The significant changes werer detected in cell morphology in group CpG2006 and CpG1668,with big cell body,obvious pigmentation,and many dendrites.Conclusions Part of the synthetic oligonucleotides is able to promote tyrosinase activity and increase cell dendrite.The research is the basis of studying vitiligo repigmentation.

关 键 词：寡核苷酸序列 毛囊 黑素细胞 

分 类 号：Q813.1[生物学—生物工程]