高脂高糖饮食结合链脲佐菌素建立2型糖尿病大鼠模型的改良  

Method Improvement for Model Establishment of Type 2 Diabetes Induced by Streptozotocin and High-carbon Hydrate-fat Diet in Rat

作　　者：蒋朝晖[1] 吕玉晶[1] 赵芳[1] 朱丽英[1] 杨国珍[1] 潘卫[1] 

机构地区：[1]贵阳医学院医学检验系,贵阳550004

出　　处：《中国比较医学杂志》2011年第1期33-35,44,87,共5页Chinese Journal of Comparative Medicine

基　　金：贵阳市科技局创新基金([2007]筑科技合同第6-14号);贵州省科学技术基金(黔科合J字[2008]2157号);贵州省优秀科技教育人才省长专项基金(黔省专合字[2006]56号);贵州省教育厅自然科学研究项目

摘　　要：目的对目前常用的高脂高糖饮食结合链脲佐菌素建(STZ)建立2型糖尿病大鼠模型的方法进行改良。方法选择40只雄性SD幼鼠,对传统方法进行改良。改良的方法包括:1.采用幼鼠(4周龄)造模;2.用实验方法确定STZ致糖尿病的亚致病剂量;3.大鼠生化指标检测根据用血量不同采取尾静脉、尾动脉和摘除眼球取血等多种取血方式。结果实验组大鼠血糖明显升高(13.7±1.57 mmol/L)。大鼠肝脏、胰腺、脑组织和肾脏等多个器官出现了病变,实验大鼠生存时间较长(9个月以上)。结论本研究完善了2型糖尿病的建模方法。Objective To modify the method of establishing type 2 diabetic model in rats with streptozotocin（STZ） and high-carbon hydrate-fat diet.Methods 40 male SD young rats were selected and the modified key points of methods were as following,1 ﹚ the rats for establishing type 2 diabetic model were 4 weeks old;2 ﹚ to determine the subclinical type 2 diabetes dose of STZ by experiments;3 ﹚ collecting blood from caudal vein,caudal artery and eyeball for the biochemistry test of rats.Results The blood glucose of rats significantly increased（13.7 ± 1.57 mmol/L）.The liver,pancreas,brain and kidney of model rats showed pathological changes.The survival time of model rats was longer（over 9 months）.Conclusions The study improves the method of establishing type 2 diabetic model.

关 键 词：2型糖尿病 模型 动物 

分 类 号：Q95-336[生物学—动物学] R332[医药卫生—人体生理学]