以2,4,6-三羟基苯乙酮为基体使用基体辅助激光解吸/电离质谱法测定核酸分子的研究  

A Study of Using 2,4,6 - Trihydroxyacetophenone as Matrix in Matrix - assisted Laser Desorption/Ionization Mass Spectrometry for DNA Detection

作　　者：黎军[1] 邓慧敏[1] 赖志辉 赵善楷 

机构地区：[1]中山大学测试中心,广州510275

出　　处：《化学学报》1999年第10期1142-1146,共5页Acta Chimica Sinica

基　　金：国家自然科学基金(29170244)资助课题

摘　　要：报道以2,4,6-三羟基苯乙酮(2,4,6-THAP)为基体用基体辅助激光解吸/电离飞行时间质谱法(MALDI-TOF-MS)测定核酸分子的研究.2,4,6-THAP解吸和电离DNA分子的效率很高,测定DNA分子d(T)_(10)的[M-H]^-分辨率可达1130.0,信噪比为366.0,检出限达5×10^(-14)mol,可以测定相对分子质量高达14800以上混合碱基组成的DNA分子.为提高基体对样品解吸/电离效率,对纯化DNA样品的方法进行了讨论.This paper reports the study on matrix - assisted laser desorption/ionization time - of - flight mass spectrometry ( MALDI - TOF - MS) of DNA, using 2,4,6 - trihydroxyacetophenone (2,4,6 -THAP) as matrix.The results show that 2,4,6 - THAP has high efficiency in desorbing and ionizing DNA molecules: for [M-H]- of d(T)10,the resolution is 1130.0, the signal - to - noise ratio is 366.0, and the detection limit is 5×10-14mol; it can measure mix - based DNA with molecular weight higher than 14800. In order to improve desorption/ionization effects, the purification methods for DNA samples are established.

关 键 词：测定 MALDI TOF MS 核酸 三羟基苯乙酮 

分 类 号：Q520.3[生物学—生物化学]