瑞舒伐他汀对猪骨髓间充质干细胞增殖、凋亡及分泌功能的影响  

Effects of rosuvastatatin on proliferation,apoptosis and secretion of porcine bone marrow mesenchymal stem cells

作　　者：潘啸东[1] 李拥军[2] 陈中璞[1] 姚玉宇[1] 马根山[2] 

机构地区：[1]东南大学医学院,南京市210009 [2]东南大学附属中大医院心脏病科

出　　处：《江苏医药》2011年第2期142-144,共3页Jiangsu Medical Journal

基　　金：江苏省"科教兴卫工程"医学重点人才项目(RC2007103)

摘　　要：目的观察不同浓度的瑞舒伐他汀在体外水平对猪骨髓间充质干细胞(BMSCs)增殖、凋亡及细胞分泌功能的影响。方法分离培养猪BMSCs,取第3代细胞随机分为对照组和不同浓度瑞舒伐他汀(0.001、0.01、0.1、1.0μmol/L)干预组。MTT比色法检测各组细胞增殖情况,膜联蛋白V-异硫氰酸荧光素/碘化丙啶(Annexin V-FITC/PI)流式细胞术检测各组抗凋亡情况,ELISA法测定细胞上清液中血管内皮生长因子(VEGF)和碱性成纤维生长因子(bFGF)含量。结果与对照组相比,瑞舒伐他汀在一定浓度范围内(0.001～0.1μmol/L)可促进BMSCs增殖,抑制其凋亡,在0.01～1.0μmol/L内增加BMSCs VEGF和bFGF的分泌(P<0.05);当瑞舒伐他汀浓度升高至1.0μmol/L时则未见促增殖和抑制凋亡的作用(P>0.05)。结论瑞舒伐他汀在一定浓度范围内可促进猪BMSCs增殖,抑制其凋亡,并对VEGF和bFGF的分泌有促进作用。Objective To investigate the effects of rosuvastatain on proliferation,apoptosis and secretion of porcine bone marrow mesenchymal stem cells （BMSCs） in vitro.Methods BMSCs were isolated and expanded.Cells of the 3rd generation were randomly divided into control group and 4 groups intervented with different concentrations of rosuvastatatin（0.001,0.01,0.1,1.0 μmol/L）.Cell proliferation was assessed by MTT colorimetry.The anti-apoptotic effects were detected by flow cytometry using Annexin V-FITC/PI assay.Levels of vascular endothelial growth factor（VEGF） and basic fibroblast growth factor（bFGF） from the supernatant were detected by ELISA.ResultsCompared to control group,rosuvastatin in a certain concentration range enhanced proliferation,inhibited cell apoptosis （0.001-0.1 μmol/L） and increased the secretions of VEGF and bFGF （0.01-1.0 μmol/L） in BMSCs （P0.05）,which was not at the concentration of 1 μmol/L（P0.05）.Conclusion Rosuvastatin in a certain concentration range could promote proliferation of BMSCs,inhibit cell apoptosis and enhance the secretions of VEGF and bFGF.

关 键 词：瑞舒伐他汀 骨髓间充质干细胞 

分 类 号：R972[医药卫生—药品]