IL-17和RORγt在类风湿关节炎CD4^+ T细胞中的表达及临床意义  

Expression and significance of IL-17 and RORγt in rheumatoid arthritis CD4^+ T cells

作　　者：吴玲[1] 李晶[1] 芮金兵[1] 陈雷[1] 尤海燕[1] 裘影影[1] 罗燕萍[1] 焦志军[1] 

机构地区：[1]江苏大学附属医院风湿病科,镇江市212001

出　　处：《江苏医药》2011年第2期156-158,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金资助项目(30872335);镇江市重点医学人才资助项目(2005009);江苏大学基金(20080008)

摘　　要：目的探讨类风湿关节炎(RA)患者外周血CD4+ T细胞中白细胞介素(IL)-17、孤核受体(RORγt)表达及意义。方法 35例RA患者分为病情活动组(A组,20例)和RA病情稳定组(B组,15例);另设健康人作对照(C组,15例)。取外周血单个核细胞(PBMC),免疫磁珠阴选CD4+ T细胞,通过流式细胞术(FCM)检测CD4+ T细胞内IL-17蛋白水平;非特异性刺激剂(A-CD3、A-CD28)刺激CD4+ T细胞后,RT-PCR检测IL-17、RORγt mRNA水平。结果与B组和C组比较,A组IL-17蛋白水平和IL-17、RORγt mRNA表达均显著增高(P<0.01),且B组各指标也高于C组(P<0.05)。A-CD3/A-CD28刺激CD4+ T细胞12h后,IL-17、RORγt mRNA表达水平较刺激24h明显增加(P<0.01),且两组各指标亦均高于未刺激组(P<0.05)。结论 RA患者外周血CD4+ T细胞中IL-17、RORγt mRNA高表达,这可能与RA病情活动相关。Objective To explore the expression and significance of interleukin（IL）-17 and retinoid-related orphan receptor γt（RORγt） in CD4＋ T cells in patients with rheumatoid arthritis（RA）.Methods Thirty-five RA patients were divided into groups of A（active RA,20 cases） and B（stable RA,15 cases）. Fifteen healthy people were taken as the controls（group C）.The protein level of IL-17 was detected by flow cytometry after isolation of untouched human CD4＋T cells from peripheral blood mononuclear cell（PBMC）.The expression levels of IL-17 and RORγt mRNA in A-CD3/A-CD28-stimulated CD4＋ T cells were determined by RT-PCR.Results Compared with groups of B and C,the expression levels of IL-17 protein and IL-17,RORγt mRNA in group A were all significantly increased（P0.01）,which were higher in group B than those in group C（P0.05）.In contrast with A-CD3/A-CD28-induced CD4＋ T cells for 24 h,the levels of IL-17 and RORγt mRNA after stimulation for 12 h were increased（P0.01）,which were all higher than those in un-stimulation group（P0.05）.Conclusion IL-17 and RORγt mRNA in RA CD4＋ T cells are highly expressed,which may be related with development and progression of RA.

关 键 词：类风湿关节炎 白细胞介素17 孤核受体 

分 类 号：R593[医药卫生—内科学]