胶原-羟磷灰石复合膜引导骨组织再生的动物实验研究  

The efficacy of collagen-hydroxyapatite composite membrane on bone regeneration

作　　者：金琼[1] 王晓敏[2] 王晓飞[1] 李旭东[2] 麻健丰[1] 

机构地区：[1]温州医学院附属口腔医院口腔修复科,温州325027 [2]四川大学生物材料工程技术研究中心,成都610064

出　　处：《华西口腔医学杂志》2011年第1期21-26,共6页West China Journal of Stomatology

摘　　要：目的探讨自制胶原-羟磷灰石(COL-HA)复合膜修复SD大鼠颅骨缺损的效果。方法在24只SD大鼠的颅顶骨上各制备4个缺损,其中左、右侧缺损区分别覆盖COL-HA复合单层致密膜(第2组)和COL-HA复合双层膜(第3组),额骨缺损区保留作为空白对照(第1组),枕骨缺损区覆盖Bio-Gide膜作为对比(第4组)。术后2、4、8、12周各处死6只大鼠取材,进行肉眼观察、X线检查、组织切片观察和新生骨量测定,对结果进行广义线性模型/析因设计方差分析和LSD-t检验。结果从术后2周开始除第1组外,其余3组均可见少量新生骨质,12周时3组缺损区由不透明硬组织封闭,并可见分解的部分膜碎片。X线显示,术后12周时第3组和第4组缺损区修复骨密度与原骨质接近,第2组稍低。新生骨量分析显示,术后初期第4组新生骨量大于其余3组,术后12周第4组与第3组和第2组的成骨量无统计学差异。结论 COL-HA复合膜能引导大鼠颅骨组织再生,且复合双层膜成骨效果要优于复合单层致密膜,其结构更有利于成骨细胞的附着和生长。Objective To investigate the efficacy of a new collagen-hydroxyapatite（COL-HA） composite membrane on bone regeneration of SD rat cranial defects.Methods Four defects were produced in the calvaria of 24 SD rats.The animals were divided into four groups：Empty defects without membrane（group 1）;defects covered by COL-HA single-layer dense membranes（group 2）;defects covered by COL-HA double-layer membranes（group 3）; defects covered by Bio-Gide membranes（group 4）.At 2,4,8 and 12 weeks after surgery,6 rats were sacrificed and the following parameters were analyzed：Macroscopic observation,X-ray examination,descriptive histology,regenerate bone quantitative histology.Statistical analysis consisted of generalized linear models/factorial design analysis of variance and LSD-t test was performed.Results Since two weeks after surgery,there were a small amount of bone regenerated in three groups except group 1.At 12 weeks after surgery,the opaque sclerous tissues filled with the defects in three groups,and residual membrane fragments still could be found.X-ray pictures showed the density of regenerate bone in group 3 and group 4 was closed to the original bone and greater than that of group 2.Quantitative analysis of regenerate bone showed that in initial stage,group 4 had more bone regeneration than the other groups（P0.05）,and at 12 weeks after surgery the differences between group 4 and group 2/group 3 had no statistically significant.Conclusion The COL-HA composite membranes can guide bone regeneration of rat cranial defects.The efficacy of bone regeneration of COL-HA double-layer membrane is superior to COL-HA single-layer dense membrane,because its property is more propitious to the adherence and proliferation of osteoblasts.

关 键 词：引导骨组织再生 胶原 羟磷灰石 骨缺损 

分 类 号：R318.08[医药卫生—生物医学工程]