姜黄素对口腔舌癌细胞增殖与转移能力影响的实验研究  

Anti-proliferative and anti-metastatic effects of curcumin on oral cancer cells

作　　者：陈教文[1] 汤亚玲[1] 刘红[1] 朱志宇[1] 吕迪[1] 耿宁[1] 陈宇[1] 

机构地区：[1]口腔疾病研究国家重点实验室,四川大学,成都610041

出　　处：《华西口腔医学杂志》2011年第1期83-86,共4页West China Journal of Stomatology

基　　金：高等学校博士点学科专项科研基金资助项目(20090181-110059)

摘　　要：目的探讨姜黄素对口腔舌癌细胞增殖与转移能力的作用及其作用机制。方法分别以0、5、10、20、30、60、100μmol·L-1姜黄素处理人舌癌细胞SCC-4 24 h,采用MTT法、侵袭实验、流式细胞仪、免疫荧光显微镜分别检测姜黄素对口腔舌癌细胞增殖与转移能力的影响。然后,采用cDNA微陈列芯片技术筛选和实时定量RT-PCR验证姜黄素所调控的相关基因。结果 MTT法检测显示:超过20μmol·L-1浓度时姜黄素明显地抑制细胞增殖。侵袭实验显示:姜黄素在20、30、60μmol·L-1时对SCC-4癌细胞的侵袭有明显抑制作用。流式细胞仪分析显示:姜黄素在20、30、60μmol·L-1的剂量下对细胞周期的分布有显著的作用,荧光显微镜观察,姜黄素(30μmol·L-1)会造成SCC-4癌细胞的细胞支架中的alpha-tubulin产生聚集的现象。cDNA微陈列芯片分析显示:87个基因可被姜黄素诱导增加;198个基因受姜黄素抑制。结论姜黄素抑制口腔舌癌的增殖和转移,其机制可能是使cdc27、EGFR sub-strate 15、PPAR-alpha和H2A histone基因表达水平降低,从而使细胞停留在细胞周期的M期,达到抗舌癌细胞增殖的作用。Objective The purpose of this article is to examine the effect of curcumin on the proliferation and metastasis of human tongue squamous cell carcinoma and analyze its mechanism.Methods SCC-4 were treated with curcumin of 0,5,10,20,30,60,100 μmol·L-1 in 24 h.MTT assay,Matrigel invasion assay,flow cytometry and flu-orescence microscopy were used to examine the effect of curcumin on the growth and metastasis of SCC-4.cDNA microarray and RT-PCR were employed to analyze the expression of genes treated by curcumin.Results The results showed that curcumin could concentration-dependently inhibit SCC-4 cell proliferation at the concentration range from 20 to 100 μmol·L-1.Furthermore,Matrigel invasion assay indicated that curcumin can reduce SCC-4 cell invasion under the dosage of 20,30,60 μmol·L-1.Flow cytometry also showed that curcumin can influence the distribution of cell cycle of SCC-4 cell with the dosage of 20,30,60 μmol·L-1.And the dosage of 30 μmol·L-1 curcumin could lead to the recruitment of alpha-tubulin.cDNA microarray showed that 87 genes were activated and 198 genes were inhibited with the effect of curcumin.These results were validated by the real time quantitative RT-PCR.Conclusion According to the results,it suggests that curcumin has the potential as the leading compound for anti-cancer proliferation and invasion in oral cancer treatment,and cdc27,EGFR substrate 15,PPAR-alpha and H2A histone may play an important role among this multiple anticancer-targeting ability.

关 键 词：舌癌 姜黄素 增殖 转移 

分 类 号：R285.5[医药卫生—中药学] R739.8[医药卫生—中医学]